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Previous issue date: 2017-02-22 / A utiliza??o de recursos naturais bot?nicos atrav?s da aplica??o da fitoterapia nos Programas de Aten??o B?sica, visando melhorar a assist?ncia ? sa?de, sobretudo em Odontologia, por meio da utiliza??o de uma tecnologia pr?tica e acess?vel a popula??o, com um custo reduzido, ? algo oportuno e inspirador. Assim sendo, demtre as esp?cies de plantas com propriedades medicinais, especialmente atividade antimicrobiana, o presente estudo se prop?s a estudar a Spondias mombin L., popularmente conhecida como caj?. Somando-se a isso o fato dos micro-organismos Staphylococcus aureus, a Pseudomonas aeruginosa e a Escherichia coli, serem citados na literatura como pat?genos oportunistas e de grande relev?ncia cl?nica para para o meio bucal. O objetivo deste estudo foi realizar a triagem fitoquimica do extrato retirado das folhas da Spondias mombin L. e avaliar, in vitro, sua a??o antimicrobiana frente ?s referidas bact?rias. A triagem fitoqu?mica dos extratos foi investigada por meio de rea??es qu?micas e pela t?cnica da cromatografia em camada delgada (CCD). Atrav?s das rea??es qu?micas foram detectados: compostos fen?licos, flavonoides, taninos e saponinas. J? o potencial do referido extrato frente a tais bact?rias foram testados atrav?s dos testes de Concentra??o Inibit?ria m?nima (CIM), Concentra??o inibit?ria m?nima de ader?ncia (CIMA) e cin?tica bactericida. Tendo nisto sido observado que o extrato S. mombin L. apresentou atividade sobre os microrganismos testados, apresentou efeito antiaderente sobre todas as bact?rias testadas, revelou que o extrato bruto foi not?vel j? nas primeiras duas horas de contato com as amostras testadas. / The use of Natural botanical resources through Phytotherapy in Basic Care Programs is able to improve the quality of healthcare in a inspiring and timely manner, especially in areas such as Dentistry, by providing low cost accessible technology to the patients. Thus, among the numerous species of plants with medicinal properties, especially antimicrobial activity, the present study proposed to study Spondias mombin L., popularly known as caj?. In addition to the fact that the microorganisms Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli, are mentioned in the literature as opportunistic pathogens and of great clinical relevance for the oral environment.The goal of this study was to carry out the phytochemical screening of the extract from the leaves of Spondias mombin L. and to evaluate, in vitro, its antimicrobial action against such bacteria. Phytochemical screening of the extracts was investigated through chemical reactions and through the thin-layer chromatography (CCD) technique. The detected compounds were: phenolic compounds, flavonoids, tannins and saponins. The potential of this extract against such bacteria were tested through the tests of Minimum Inhibitory Concentration (MIC), Minimum Inhibitory Adhesion Concentration (CIMA) and bactericidal kinetics. It was observed that the extract S. mombin L. showed activity on the tested microorganisms, and a non-sticky effect on all the bacteria tested. It also revealed that the crude extract was remarkable mature within the first two hours of contact with the samples tested.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/23619 |
| Date | 22 February 2017 |
| Creators | Alves, Rivanaldo Adriano Holanda |
| Contributors | 87808838420, http://lattes.cnpq.br/4462076152744400, Dametto, F?bio Roberto, 13119344800, http://lattes.cnpq.br/3622339979189162, Gad? Neto, C?cero Rom?o, 50430947453, http://lattes.cnpq.br/0060206360854211, Lins, Ruthineia Di?genes Alves Uchoa |
| Publisher | PROGRAMA DE P?S-GRADUA??O EM SA?DE COLETIVA, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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