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Immunological and molecular studies of shrimp allergens.

by Chow Wing Kuen. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1993. / Includes bibliographical references (leaves 94-109). / Abstract --- p.i / Acknowledgements --- p.iii / Table of contents --- p.v / List of Tables --- p.viii / List of Figures --- p.ix / Abbreviations --- p.xi / Chapter Chapter 1 --- General Introduction --- p.1 / Chapter Chapter 2 --- Literature review / Chapter 2.1 --- Hypersensitivity to Crustacea --- p.3 / Chapter 2.2 --- Characterization of allergens of shrimp --- p.10 / Chapter 2.3 --- Cross reactivity of crustacean allergens --- p.18 / Chapter 2.4 --- Molecular cloning and expression of allergens --- p.22 / Chapter Chapter 3 --- Immunological characterization of shrimp allergens / Chapter 3.1 --- Introduction --- p.26 / Chapter 3.2 --- Material and Methods / Chapter 3.2.1 --- Animals --- p.28 / Chapter 3.2.2 --- Sera --- p.28 / Chapter 3.2.3 --- Shrimp tissue extract --- p.29 / Chapter 3.2.4 --- Dot blotting --- p.29 / Chapter 3.2.5 --- Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) --- p.30 / Chapter 3.2.6 --- Immunoblotting --- p.32 / Chapter 3.3.7 --- Immunological detection of IgE binding proteins --- p.32 / Chapter 3.3 --- Results / Chapter 3.3.1 --- Detection of allergens in raw and cooked shrimp muscle extract --- p.35 / Chapter 3.3.2 --- Detection of allergens in hepatopancreas and ovary of the shrimp --- p.38 / Chapter 3.3.3 --- Detection of allergens in boiling shrimp fluid --- p.42 / Chapter 3.3.4 --- Detection of allergens in dried shrimp --- p.48 / Chapter 3.3.5 --- Reactivity of IgE from the shrimp- sensitive subjects with extracts from different species of penaeid shrimp --- p.48 / Chapter 3.3.6 --- Reactivity of IgE from the shrimp- sensitive subjects with muscle extracts of crustaceans and mollusks --- p.52 / Chapter 3.4 --- Discussion --- p.55 / Chapter Chapter 4 --- "Construction and immunoscreening of the cDNA library from muscle of the shrimp, Metapenaeus ensis" / Chapter 4.1 --- Introduction --- p.63 / Chapter 4.2 --- Materials and Methods / Chapter 4.2.1 --- Animals --- p.66 / Chapter 4.2.2 --- Sera --- p.66 / Chapter 4.2.3 --- Controlling ribonuclease activity --- p.66 / Chapter 4.2.4 --- Isolation of total RNA --- p.67 / Chapter 4.2.5 --- Isolation of mRNA / Chapter 4.2.5.1 --- Oligo-d(T) cellulose chromatography --- p.68 / Chapter 4.2.5.2 --- Magnetic separation --- p.70 / Chapter 4.2.6 --- Synthesis of double stranded cDNA --- p.71 / Chapter 4.2.7 --- Generation of EcoRI cohesive ends on cDNA --- p.72 / Chapter 4.2.8 --- Ligation of cDNA with λgtll vector --- p.74 / Chapter 4.2.9 --- In vitro packaging --- p.74 / Chapter 4.2.10 --- Titration of phage library --- p.75 / Chapter 4.2.11 --- Absorption of anti-E.coli antibodies --- p.76 / Chapter 4.2.12 --- Immunoscreening of the shrimp muscle cDNA library --- p.77 / Chapter 4.3 --- Results --- p.80 / Chapter 4.4 --- Discussion --- p.89 / Chapter Chapter 5 --- General conclusion --- p.92 / References --- p.94

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_319132
Date January 1993
ContributorsChow, Wing Kuen., Chinese University of Hong Kong Graduate School. Division of Biology.
PublisherChinese University of Hong Kong
Source SetsThe Chinese University of Hong Kong
LanguageEnglish
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xi, 109 leaves : ill. (chiefly mounted) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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