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Previous issue date: 2017-03-30 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Potatoes (Solanum tuberosum L.) are the third most consumed crop in the world,
after rice and wheat. Among the diseases affecting potato, the blackleg and tuber soft
rot, caused by phytobacteria Pectobacterium spp., lead to significant losses in the yield
crop. Several studies have been exploring the use of plant defense inducers as a
strategy to control plant diseases. The use of plant growth-promoting rhizobacteria
(PGPR) in agriculture can lead to plant growth and enhancement of plant defense
through the promotion of induced systemic resistance (ISR). However, the mechanisms
involved in promoting ISR are still poorly understood. This study aimed to screen
rhizobacteria of the genus Streptomyces with capacity to promote plant growth and
induce Solanum tuberosum innate immunity. To achieve these objectives, we evaluated:
i) the ability of Streptomyces isolates to produce auxin (3-indoleacetic acid), ACC
deaminase (1-aminocyclopropane-1-carboxylic acid deaminase) and siderophores; ii)
their capacity to promote the growth of potato plants; iii) the induction of resistance in
potato plants challenged with Pectobacterium carotovorum subsp. brasiliensis and iv)
the expression of genes related to defense pathways in S. tuberosum, promoted by
Streptomyces and P. carotovorum. Results indicated that the CLV163 isolate presents
PGPR features, such as high auxin and siderophores production, and promoted plant
defense against P. carotovorum. Although CLV145 showed the highest auxin and
siderophores production, it reduced the shoot dry mass and was inefficient in promoting
plant defense. Moreover, the ability of Streptomyces in producing ACC deaminase was
not critical for promoting plant growth. The CLV163 isolate induced a priming state in
potato plants that has occurred through the activation of the AS and ET pathways, and
its interaction with S. tuberosum plants did not impair the plant growth. / A batata (Solanum tuberosum L.) ? a terceira cultura agr?cola mais consumida
no mundo, ficando atr?s somente do arroz e do trigo. Dentre as diversas doen?as que
afetam esta cultura, a canela-preta e a podrid?o-mole, causadas pela fitobact?ria
Pectobacterium spp., levam a importantes perdas na produ??o. Neste contexto, v?rios
trabalhos v?m explorando o uso de indutores da defesa vegetal como estrat?gia para o
controle de doen?as. O uso de rizobact?rias promotoras do crescimento vegetal (PGPR)
na agricultura pode promover tanto o crescimento quanto o aumento da defesa das
plantas atrav?s da indu??o da resist?ncia sist?mica induzida (ISR). Contudo, ainda s?o
pouco conhecidos os mecanismos envolvidos na promo??o da ISR. Este estudo visou
selecionar rizobact?rias do g?nero Streptomyces com caracter?sticas de promotoras de
crescimento e indutoras da imunidade inata de Solanum tuberosum. Para tanto, foram
avaliadas: i) a capacidade de isolados de Streptomyces produzirem auxina (?cido 3-
indolac?tico), ACC desaminase (?cido 1-aminociclopropano-1-carbox?lico desaminase)
e sider?foros; ii) a capacidade destas rizobact?rias em promover o crescimento das
plantas; iii) a promo??o da resist?ncia em plantas de batata desafiadas com
Pectobacterium carotovorum subsp. brasiliensis e iv) a express?o de genes
relacionados a vias de sinaliza??o de respostas de defesa em S. tuberosum, promovida
por Streptomyces sp e a fitobact?ria P. carotovorum. Os resultados indicam que o
isolado CLV163 apresenta caracter?sticas de PGPR, sendo capaz de produzir auxina e
sider?foros, al?m de promover o aumento da resist?ncia das plantas contra P.
carotovorum. Contudo, a rizobact?ria CLV145, com a maior produ??o de auxina e
sider?foros, causou a diminui??o da mat?ria seca de parte a?rea e n?o promoveu a
defesa das plantas. A capacidade dos Streptomyces em produzirem ACC desaminase
n?o foi determinante para a promo??o de crescimento vegetal. O isolado CLV163
induziu um estado de priming nas plantas de S. tuberosum L. atrav?s da ativa??o das
vias do AS e ET e a sua intera??o com as plantas de batata n?o comprometeu o
crescimento vegetal.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/7899 |
| Date | 30 March 2017 |
| Creators | Vilches, Patr?cia Fernanda da Silva |
| Contributors | Astarita, Leandro, Silva, Renata Medina da |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Biologia Celular e Molecular, PUCRS, Brasil, Escola de Ci?ncias |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 8198246930096637360, 500, 500, 600, -1634559385931244697, 2075167498588264571 |
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