Delta-8-tetrahydrocannabinol (THC), although less potent than THC, can still reduce memory cognition and anxiety. However, Delta-8 can have adverse effects at high doses, like inducing anxiety, confusion, and hallucinations. The brain of Danio rerio (zebrafish) resembles human neuroanatomical and neurochemical pathways and demonstrates robust behavioral phenotypes, making zebrafish an excellent model organism for memory and anxiety. Subjects will be fed 5mg of Delta 8-THC before entering the experimental apparatus. Fish for both memory and anxiety tests will be observed and recorded through Noldus Ethovision Software. For testing memory, five female and five male zebrafish will be individually placed in a three-compartment memory tank. One side of the memory tank will have a star shape, while the other side will have a circle shape. About .83mg of commercial fish flakes will be administered to the fish on the circle side in the first trial, then on the star side in the second trial, and will continue on either side for 20 trials. Correct responses will be scored when the fish are present on the side of the tank used to present the fish flakes. We hypothesize that there will be fewer correct responses for fish fed with Delta-8-THC than the control. For testing anxiety, ten male and female zebrafish will be fed 5mg of Delta 8-THC before entering the diving tank. Typically, zebrafish experiencing anxiety will stay at the bottom of the diving tank, but if they are relaxed, they will be at the top. We hypothesize that fish fed with Delta-8-THC will spend more time at the top of the diving tank than the control.
| Identifer | oai:union.ndltd.org:ETSU/oai:dc.etsu.edu:asrf-2075 |
| Date | 25 April 2023 |
| Creators | Biragbara, Dornu, Azizi, Ava, McGrew, Lori |
| Publisher | Digital Commons @ East Tennessee State University |
| Source Sets | East Tennessee State University |
| Detected Language | English |
| Type | text |
| Source | Appalachian Student Research Forum |
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