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Previous issue date: 2015-03-05 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Potato is currently the third most consumed food crop after rice and wheat, and it is a valuable resource for alleviating poverty in undeveloped countries. However, potato crop fields are attacked by numerous pests and pathogens, which represent a threat to the global potato production. Unlike animals, plants do not possess an adaptive immune system. Therefore, each individual plant cell needs to be able to perceive the pathogen, release signals to neighbor cells, and produce a defense response. Plant cells recognize the pathogen through Pattern Recognition Receptors (PRRs), triggering a signaling cascade that results in the activation of defense-related genes, which characterize the occurrence of Systemic Acquired Resistance (SAR). Among the factors involved in the regulation of defense-related genes, salicylic acid is widely known as an elicitor of plant defense against biotrophic pathogens.Besides this hormone, it has been demonstrated that a bacterial extract of Xanthomonas axonopodis pv. citri (referred to as XTH) is capable of inducing resistance against pectolytic bacteria via poorly understood mechanisms. Considering the different signaling pathways involved in plant defense, we intended to investigate the occurrence of systemic resistance in Solanum tuberosum plants treated with XTH or salicylic acid, by analyzing the expression of PR-1b, PR-2, ChtA, PAL, Pin2, JAZ1/TIFY10A, and ERF1 genes. Our results suggest that XTH is capable of inducing systemic resistance in potato plants via concomitant activation of the salicylic acid, jasmonic acid, and ethylene signaling pathways. / A batata ? atualmente o terceiro alimento mais consumido no mundo, depois do arroz e do trigo, al?m de ser um recurso importante no combate ? fome em pa?ses subdesenvolvidos. Entretanto, plantas de batata s?o atacadas por diversas pragas e pat?genos, que representam uma amea?a ? produ??o global de tub?rculos. Ao contr?rio dos animais, as plantas n?o possuem um sistema imune adaptativo. Dessa forma, as plantas dependem que cada c?lula, individualmente, tenha a capacidade de perceber o pat?geno, sinalizar ?s c?lulas vizinhas e produzir uma resposta de defesa. As c?lulas vegetais percebem o contato do pat?geno atrav?s de Receptores de Reconhecimento de Padr?es (PRRs), desencadeando cascatas de sinaliza??o que levam ? ativa??o de genes de defesa, os quais caracterizam a ocorr?ncia de Resist?ncia Sist?mica Adquirida (SAR). Dentre os fatores envolvidos na regula??o de genes relacionados ? defesa, o ?cido salic?lico ? amplamente conhecido como um eliciador da defesa vegetal contra pat?genos biotr?ficos.Al?m deste horm?nio, foi demonstrado que o extrato bacteriano de Xanthomonas axonopodis pv. citri (denominado de indutor XTH) possui a caracter?stica de induzir resist?ncia contra fitobact?rias pectol?ticas atrav?s de mecanismos ainda pouco conhecidos. Considerando as diferentes vias de sinaliza??o envolvidas na defesa vegetal, pretendeu-se investigar a ocorr?ncia de resist?ncia sist?mica em plantas de Solanum tuberosum tratadas com indutor XTH ou ?cido salic?lico, atrav?s da an?lise transcricional dos genes PR-1b, PR-2, ChtA, PAL, Pin2, JAZ1/TIFY10A e ERF1. Os resultados sugerem que o indutor XTH tem a capacidade de induzir resist?ncia sist?mica em plantas de batata atrav?s da ativa??o concomitante das vias de sinaliza??o do ?cido salic?lico, jasmonato e etileno.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/6229 |
| Date | 05 March 2015 |
| Creators | Sartor, Tiago |
| Contributors | Astarita , Leandro Vieira |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Biologia Celular e Molecular, PUCRS, Brasil, Faculdade de Bioci?ncias |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 8198246930096637360, 600, 600, 600, 600, 36528317262667714, -1634559385931244697, 2075167498588264571 |
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