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Pyrimidine Nucleoside Metabolism in Pseudomonads and Enteric Bacteria

Metabolic differences in the strategies used for pyrimidine base and nucleoside salvage were studied in the pseudomonads and enteric bacteria. Fluoro--analogs were used to select mutant strains of E. coli, S. typhimurium, P. putida, and P. aeruginosa blocked in one or more of the uracil and uridine salvage enzymes. HPLC analysis of cell-free extracts from wild-type and mutant strains examined the effectiveness of the selections. Evidence was found for cytidine kinase in Pseudomonas and for an activity that converted uracil compounds to cytosine compounds. Using media supplemented with 150 μg of orotic acid per ml, P. putida SOC 1, a Pyr, upp mutant which utilizes orotic acid as a pyrimidine source was isolated for the first time in any study.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc500941
Date12 1900
CreatorsScott, Allelia Worrall
ContributorsO'Donovan, Gerard A., Benjamin, Robert C., Campbell, A. C.
PublisherUniversity of North Texas
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatviii, 92 leaves: ill., Text
RightsPublic, Scott, Allelia Worrall, Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved.

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