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Studies on regulatory mechanisms of electrolyte transport in the rat epididymis.

by Zhao Wei. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1996. / Includes bibliographical references (leaves 58-74). / Declaration --- p.i / Acknowledgements --- p.iii / Summary --- p.iii / Abbreviations --- p.v / Chapter CHAPTER 1 --- LITERATURE REVIEW --- p.1 / Chapter 1.1 --- Introduction --- p.1 / Chapter 1.2 --- Anatomy and functions of the epididymis --- p.1 / Chapter 1.2.1 --- Anatomy of the epididymis --- p.1 / Chapter 1.2.2 --- Histology and cytology of the epididymis --- p.2 / Chapter 1.2.3 --- Innervation and vasculation of the epididymis --- p.4 / Chapter 1.2.4 --- Sperm maturation and fertility in the epididymis --- p.6 / Chapter 1.2.5 --- Absorptive and secretory process of the epididymal epithelium --- p.8 / Chapter 1.2.6 --- Regulation of epididymal anion secretion --- p.9 / Chapter 1.3 --- Project aims --- p.12 / Chapter CHAPTER 2 --- METHODS AND MATERIALS --- p.13 / Chapter 2.1 --- Tissue preparation --- p.13 / Chapter 2.2 --- Light microscopic immunohistochemistry --- p.13 / Chapter 2.3 --- Cell culture and radioimmunoassay --- p.14 / Chapter 2.4 --- Cell culture and short -circuit current (Isc) measurement --- p.15 / Chapter 2.5 --- Reagents --- p.16 / Chapter 2.6 --- Statistical analysis --- p.16 / Chapter CHAPTER 3 --- LOCALIZATION AND DISTRIBUTION OF angiotensin ii in the rat epididymis --- p.17 / Chapter 3.1 --- Introduction --- p.17 / Chapter 3.2 --- Results --- p.18 / Chapter 3.2.1 --- Immunohistochemical localization of angiotensin II --- p.18 / Chapter 3.2.2 --- Angiotensin II activity measured by radioimmunoassay --- p.31 / Chapter 3.3 --- Discussion --- p.31 / Chapter CHAPTER 4 --- CELLULAR MECHANISM OF cAMP-DEPENDENTE BICARBONATE SECRETION IN RAT EPIDIDYMAL EPITHELIUM --- p.38 / Chapter 4.1 --- Introduction --- p.38 / Chapter 4.2 --- Results --- p.40 / Chapter 4.2.1 --- Effects of Cl and HCO3- on cyt-cAMP stimulated Isc in rat epididymal epithelia --- p.40 / Chapter 4.2.2 --- Effects of inhibitors on the cpt-cAMP stimulated Iscin HCO3- free solution --- p.41 / Chapter 4.2.3 --- Effects of inhibitors on the cpt-cAMP stimulated Isc in Cl-free solution --- p.41 / Chapter 4.2.4 --- Effect of hexokinase on cyt-cAMP induced Isc --- p.48 / Chapter 4.3 --- Discussion --- p.49 / Chapter CHAPTER 5 --- CONCLUSION --- p.55 / References --- p.58 / Publications --- p.74

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_321700
Date January 1996
ContributorsZhao, Wei., Chinese University of Hong Kong Graduate School. Division of Basic Medical Sciences.
PublisherChinese University of Hong Kong
Source SetsThe Chinese University of Hong Kong
LanguageEnglish
Detected LanguageEnglish
TypeText, bibliography
Formatprint, vi, 74 leaves : ill. (some col., some mounted) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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