by Anskar Yu-hung Leung. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1992. / Includes bibliographical references (leaves 147-163). / Chapter Section I --- Literature review / Chapter Chapter I.1. --- The epididymis - its structures and functions --- p.1 / Chapter Chapter I.2. --- Cellular mechanisms of transepithelial electrolyte transport in epididymis and other exocrine tissues --- p.5 / Chapter Chapter I.3 --- Signal transduction mechanism of chloride secretion in epididymis and other exocrine tissue --- p.11 / Chapter Chapter I.4 --- Significance of chloride secretion by the epididymal epithelium and the objectives of the study --- p.17 / Chapter Section II --- General methods / Chapter Chapter II.1. --- Tissue culture from the rat cauda epididymis --- p.20 / Chapter Chapter II.2. --- The short-circuit current technique --- p.30 / Chapter Chapter II.3. --- The immunofluorescence technique --- p.39 / Chapter Chapter II.4. --- Intracellular adenosine 3':5' cyclic monophosphate (cAMP) measurement --- p.43 / Chapter Chapter II.5 --- Intracellular Ca2+ measurement using the microfluorimetric technique --- p.49 / Chapter Section III --- Results / Chapter Chapter III.1. --- Studies on the neural and humoral controls of eletrogenic chloride secretion in rat epididymis --- p.55 / Chapter Chapter III.2. --- Local control of electrogenic chloride secretion in rat epididymis 226}0ؤThe role of the calcitonin gene-related peptide --- p.80 / Chapter Chapter III.3. --- Characterization of intracellular Ca2+ store using ATP as the calcium mobilizing agonist --- p.94 / Chapter Chapter III.4. --- Ca2+ handling mechanisms in single cultured rat epididymal cells --- p.106 / Chapter Chapter III.5. --- Studies on the effector process in the stimulus-secretion coupling- characterization of apical Cl- conductance in cultured rat cauda epididmal cells --- p.114 / Chapter Chapter III.6. --- Effects of secretory agonists on transepithelial Cl- transport and intracellular Ca2+ concentration in cultured human epididymal epithelium --- p.132 / Chapter Section IV --- General discussion --- p.142 / Chapter Section V --- References --- p.147 / Appendix --- p.164

by Zhao Wei. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1996. / Includes bibliographical references (leaves 58-74). / Declaration --- p.i / Acknowledgements --- p.iii / Summary --- p.iii / Abbreviations --- p.v / Chapter CHAPTER 1 --- LITERATURE REVIEW --- p.1 / Chapter 1.1 --- Introduction --- p.1 / Chapter 1.2 --- Anatomy and functions of the epididymis --- p.1 / Chapter 1.2.1 --- Anatomy of the epididymis --- p.1 / Chapter 1.2.2 --- Histology and cytology of the epididymis --- p.2 / Chapter 1.2.3 --- Innervation and vasculation of the epididymis --- p.4 / Chapter 1.2.4 --- Sperm maturation and fertility in the epididymis --- p.6 / Chapter 1.2.5 --- Absorptive and secretory process of the epididymal epithelium --- p.8 / Chapter 1.2.6 --- Regulation of epididymal anion secretion --- p.9 / Chapter 1.3 --- Project aims --- p.12 / Chapter CHAPTER 2 --- METHODS AND MATERIALS --- p.13 / Chapter 2.1 --- Tissue preparation --- p.13 / Chapter 2.2 --- Light microscopic immunohistochemistry --- p.13 / Chapter 2.3 --- Cell culture and radioimmunoassay --- p.14 / Chapter 2.4 --- Cell culture and short -circuit current (Isc) measurement --- p.15 / Chapter 2.5 --- Reagents --- p.16 / Chapter 2.6 --- Statistical analysis --- p.16 / Chapter CHAPTER 3 --- LOCALIZATION AND DISTRIBUTION OF angiotensin ii in the rat epididymis --- p.17 / Chapter 3.1 --- Introduction --- p.17 / Chapter 3.2 --- Results --- p.18 / Chapter 3.2.1 --- Immunohistochemical localization of angiotensin II --- p.18 / Chapter 3.2.2 --- Angiotensin II activity measured by radioimmunoassay --- p.31 / Chapter 3.3 --- Discussion --- p.31 / Chapter CHAPTER 4 --- CELLULAR MECHANISM OF cAMP-DEPENDENTE BICARBONATE SECRETION IN RAT EPIDIDYMAL EPITHELIUM --- p.38 / Chapter 4.1 --- Introduction --- p.38 / Chapter 4.2 --- Results --- p.40 / Chapter 4.2.1 --- Effects of Cl and HCO3- on cyt-cAMP stimulated Isc in rat epididymal epithelia --- p.40 / Chapter 4.2.2 --- Effects of inhibitors on the cpt-cAMP stimulated Iscin HCO3- free solution --- p.41 / Chapter 4.2.3 --- Effects of inhibitors on the cpt-cAMP stimulated Isc in Cl-free solution --- p.41 / Chapter 4.2.4 --- Effect of hexokinase on cyt-cAMP induced Isc --- p.48 / Chapter 4.3 --- Discussion --- p.49 / Chapter CHAPTER 5 --- CONCLUSION --- p.55 / References --- p.58 / Publications --- p.74

published_or_final_version / Physiology / Doctoral / Doctor of Philosophy

published_or_final_version / Physiology / Doctoral / Doctor of Philosophy

by Wai-on Fu. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1993. / Includes bibliographical references (leaves 88-95). / Chapter Chapter I --- Introduction --- p.1 / Chapter I .1 --- Structure and functions of the epididymis --- p.1 / Chapter I.2 --- Cellular mechanisms for electrolyte secretion --- p.3 / Chapter I.3 --- Second-messenger modulation of chloride secretion in epididymis --- p.5 / Chapter I.3.1 --- Cyclic AMP pathway --- p.5 / Chapter I.3.2 --- Ca2+ as second messenger --- p.6 / Chapter I.4 --- Pathophysiology of electrolyte transport in the epididymis --- p.6 / Chapter I.5 --- Objectives of the study --- p.9 / Chapter Chapter II. --- Materials and Methods --- p.10 / Chapter II. 1 --- Materials --- p.10 / Chapter II. 1.1 --- Culture media and enzyme --- p.10 / Chapter II. 1.2. --- Drugs --- p.10 / Chapter II. 1.3 --- Chemicals --- p.11 / Chapter II. 1.4. --- Animals and human tissue --- p.11 / Chapter II.2 --- Preparation of solutions --- p.12 / Chapter II. 3. --- Preparation of cultured cells --- p.12 / Chapter II.3 .1 --- Culture of rat epididymal epithelial cells --- p.12 / Chapter II.3.2 --- Cultured of human epididymal epithelial cells --- p.16 / Chapter II.4. --- Patch-Clamp technique --- p.21 / Chapter II.4.1 --- Electrode --- p.23 / Chapter II.4.2 --- Pulling of electrode --- p.23 / Chapter II.4.3 --- Coating of electrode --- p.23 / Chapter II.4.4 --- Polishing of the electrode --- p.24 / Chapter II.4.5 --- Filling of the electrodes --- p.26 / Chapter II.4.6 --- Mounting of Electrode to the Headstage Pipette Holder --- p.26 / Chapter II.4.7 --- Electrical Isolation --- p.28 / Chapter II.4.8 --- Vibration Isolation --- p.28 / Chapter II.4.9 --- "Formation of ""Giga-seal"" and Whole-cell configuration" --- p.28 / Chapter II.4.10 --- Correction for liquid junction potential --- p.30 / Chapter II.4.11 --- "Data Acquisition and Analyses," --- p.32 / Chapter II.4.12 --- Statistics --- p.34 / Chapter Chapter III. --- Results --- p.35 / Anion Secretion in human epididymal cells --- p.35 / Chapter III. 1 --- Whole-cell current in human epididymal cells --- p.35 / Chapter III. 2 --- Effect of adrenergic receptor blockers on whole-cell current --- p.38 / Chapter III.3 --- Effect of inhibitors of the cyclic AMP pathway --- p.42 / Chapter III.4 --- Effect of altering intracellular calcium concentration --- p.42 / Anion secretion in rat epididymal cells --- p.46 / Chapter III. 5 --- Effect of cAMP on whole cell C1- current in rat epididymis --- p.46 / Chapter III.6 --- Effect of ionomycin on whole cell C1- current --- p.53 / Chapter III.7 --- Differences between cAMP- and ionophore- dependent C1- current --- p.57 / Chapter III. 8 --- The swelling-induced whole-cell currents --- p.63 / Chapter III.9 --- The swelling-induced current was mainly C1- selective --- p.65 / Chapter III. 10 --- Anion selectivity of the swelling-induced C1- current --- p.68 / Chapter III. 11 --- Inhibition of the swelling-induced C1- conductance by anion channel blockers --- p.68 / Chapter III. 12 --- The role of Ca2+ and cAMP in swelling-induced C1- conductance --- p.74 / Chapter Chapter IV. --- Discussion --- p.79 / Signal transduction mechanism of adrenaline stimulated C1- current in human epididymal cell --- p.79 / Anion secretion in rat epididymal cell The role of Ca2+ and cAMP --- p.83 / Chapter Chapter V. --- Reference --- p.88

published_or_final_version / Physiology / Master / Master of Philosophy