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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Neurohumoral and local controls of the electrogenic chloride secretion in rat and human epididymis with reference to the signal transduction mechanisms.

January 1992 (has links)
by Anskar Yu-hung Leung. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1992. / Includes bibliographical references (leaves 147-163). / Chapter Section I --- Literature review / Chapter Chapter I.1. --- The epididymis - its structures and functions --- p.1 / Chapter Chapter I.2. --- Cellular mechanisms of transepithelial electrolyte transport in epididymis and other exocrine tissues --- p.5 / Chapter Chapter I.3 --- Signal transduction mechanism of chloride secretion in epididymis and other exocrine tissue --- p.11 / Chapter Chapter I.4 --- Significance of chloride secretion by the epididymal epithelium and the objectives of the study --- p.17 / Chapter Section II --- General methods / Chapter Chapter II.1. --- Tissue culture from the rat cauda epididymis --- p.20 / Chapter Chapter II.2. --- The short-circuit current technique --- p.30 / Chapter Chapter II.3. --- The immunofluorescence technique --- p.39 / Chapter Chapter II.4. --- Intracellular adenosine 3':5' cyclic monophosphate (cAMP) measurement --- p.43 / Chapter Chapter II.5 --- Intracellular Ca2+ measurement using the microfluorimetric technique --- p.49 / Chapter Section III --- Results / Chapter Chapter III.1. --- Studies on the neural and humoral controls of eletrogenic chloride secretion in rat epididymis --- p.55 / Chapter Chapter III.2. --- Local control of electrogenic chloride secretion in rat epididymis 226}0ؤThe role of the calcitonin gene-related peptide --- p.80 / Chapter Chapter III.3. --- Characterization of intracellular Ca2+ store using ATP as the calcium mobilizing agonist --- p.94 / Chapter Chapter III.4. --- Ca2+ handling mechanisms in single cultured rat epididymal cells --- p.106 / Chapter Chapter III.5. --- Studies on the effector process in the stimulus-secretion coupling- characterization of apical Cl- conductance in cultured rat cauda epididmal cells --- p.114 / Chapter Chapter III.6. --- Effects of secretory agonists on transepithelial Cl- transport and intracellular Ca2+ concentration in cultured human epididymal epithelium --- p.132 / Chapter Section IV --- General discussion --- p.142 / Chapter Section V --- References --- p.147 / Appendix --- p.164
2

Studies on regulatory mechanisms of electrolyte transport in the rat epididymis.

January 1996 (has links)
by Zhao Wei. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1996. / Includes bibliographical references (leaves 58-74). / Declaration --- p.i / Acknowledgements --- p.iii / Summary --- p.iii / Abbreviations --- p.v / Chapter CHAPTER 1 --- LITERATURE REVIEW --- p.1 / Chapter 1.1 --- Introduction --- p.1 / Chapter 1.2 --- Anatomy and functions of the epididymis --- p.1 / Chapter 1.2.1 --- Anatomy of the epididymis --- p.1 / Chapter 1.2.2 --- Histology and cytology of the epididymis --- p.2 / Chapter 1.2.3 --- Innervation and vasculation of the epididymis --- p.4 / Chapter 1.2.4 --- Sperm maturation and fertility in the epididymis --- p.6 / Chapter 1.2.5 --- Absorptive and secretory process of the epididymal epithelium --- p.8 / Chapter 1.2.6 --- Regulation of epididymal anion secretion --- p.9 / Chapter 1.3 --- Project aims --- p.12 / Chapter CHAPTER 2 --- METHODS AND MATERIALS --- p.13 / Chapter 2.1 --- Tissue preparation --- p.13 / Chapter 2.2 --- Light microscopic immunohistochemistry --- p.13 / Chapter 2.3 --- Cell culture and radioimmunoassay --- p.14 / Chapter 2.4 --- Cell culture and short -circuit current (Isc) measurement --- p.15 / Chapter 2.5 --- Reagents --- p.16 / Chapter 2.6 --- Statistical analysis --- p.16 / Chapter CHAPTER 3 --- LOCALIZATION AND DISTRIBUTION OF angiotensin ii in the rat epididymis --- p.17 / Chapter 3.1 --- Introduction --- p.17 / Chapter 3.2 --- Results --- p.18 / Chapter 3.2.1 --- Immunohistochemical localization of angiotensin II --- p.18 / Chapter 3.2.2 --- Angiotensin II activity measured by radioimmunoassay --- p.31 / Chapter 3.3 --- Discussion --- p.31 / Chapter CHAPTER 4 --- CELLULAR MECHANISM OF cAMP-DEPENDENTE BICARBONATE SECRETION IN RAT EPIDIDYMAL EPITHELIUM --- p.38 / Chapter 4.1 --- Introduction --- p.38 / Chapter 4.2 --- Results --- p.40 / Chapter 4.2.1 --- Effects of Cl and HCO3- on cyt-cAMP stimulated Isc in rat epididymal epithelia --- p.40 / Chapter 4.2.2 --- Effects of inhibitors on the cpt-cAMP stimulated Iscin HCO3- free solution --- p.41 / Chapter 4.2.3 --- Effects of inhibitors on the cpt-cAMP stimulated Isc in Cl-free solution --- p.41 / Chapter 4.2.4 --- Effect of hexokinase on cyt-cAMP induced Isc --- p.48 / Chapter 4.3 --- Discussion --- p.49 / Chapter CHAPTER 5 --- CONCLUSION --- p.55 / References --- p.58 / Publications --- p.74
3

Biophysical and biochemical studies of anion secretion in the rat epididymis

黃景祥, Huang, King-chong, Stephen Joseph. January 1990 (has links)
published_or_final_version / Physiology / Doctoral / Doctor of Philosophy
4

Studies on the secretion of macromolecules by the mammalianepididymis, their interaction with spermatozoa and their roles insperm maturation

曾潤福, Tsang, Yun-fuk, Angus. January 1983 (has links)
published_or_final_version / Physiology / Doctoral / Doctor of Philosophy
5

Studies on the role of membrane conductance changes in electrolyte secretion and volume regulation in the cultured rat and human epididymal cells.

January 1993 (has links)
by Wai-on Fu. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1993. / Includes bibliographical references (leaves 88-95). / Chapter Chapter I --- Introduction --- p.1 / Chapter I .1 --- Structure and functions of the epididymis --- p.1 / Chapter I.2 --- Cellular mechanisms for electrolyte secretion --- p.3 / Chapter I.3 --- Second-messenger modulation of chloride secretion in epididymis --- p.5 / Chapter I.3.1 --- Cyclic AMP pathway --- p.5 / Chapter I.3.2 --- Ca2+ as second messenger --- p.6 / Chapter I.4 --- Pathophysiology of electrolyte transport in the epididymis --- p.6 / Chapter I.5 --- Objectives of the study --- p.9 / Chapter Chapter II. --- Materials and Methods --- p.10 / Chapter II. 1 --- Materials --- p.10 / Chapter II. 1.1 --- Culture media and enzyme --- p.10 / Chapter II. 1.2. --- Drugs --- p.10 / Chapter II. 1.3 --- Chemicals --- p.11 / Chapter II. 1.4. --- Animals and human tissue --- p.11 / Chapter II.2 --- Preparation of solutions --- p.12 / Chapter II. 3. --- Preparation of cultured cells --- p.12 / Chapter II.3 .1 --- Culture of rat epididymal epithelial cells --- p.12 / Chapter II.3.2 --- Cultured of human epididymal epithelial cells --- p.16 / Chapter II.4. --- Patch-Clamp technique --- p.21 / Chapter II.4.1 --- Electrode --- p.23 / Chapter II.4.2 --- Pulling of electrode --- p.23 / Chapter II.4.3 --- Coating of electrode --- p.23 / Chapter II.4.4 --- Polishing of the electrode --- p.24 / Chapter II.4.5 --- Filling of the electrodes --- p.26 / Chapter II.4.6 --- Mounting of Electrode to the Headstage Pipette Holder --- p.26 / Chapter II.4.7 --- Electrical Isolation --- p.28 / Chapter II.4.8 --- Vibration Isolation --- p.28 / Chapter II.4.9 --- "Formation of ""Giga-seal"" and Whole-cell configuration" --- p.28 / Chapter II.4.10 --- Correction for liquid junction potential --- p.30 / Chapter II.4.11 --- "Data Acquisition and Analyses," --- p.32 / Chapter II.4.12 --- Statistics --- p.34 / Chapter Chapter III. --- Results --- p.35 / Anion Secretion in human epididymal cells --- p.35 / Chapter III. 1 --- Whole-cell current in human epididymal cells --- p.35 / Chapter III. 2 --- Effect of adrenergic receptor blockers on whole-cell current --- p.38 / Chapter III.3 --- Effect of inhibitors of the cyclic AMP pathway --- p.42 / Chapter III.4 --- Effect of altering intracellular calcium concentration --- p.42 / Anion secretion in rat epididymal cells --- p.46 / Chapter III. 5 --- Effect of cAMP on whole cell C1- current in rat epididymis --- p.46 / Chapter III.6 --- Effect of ionomycin on whole cell C1- current --- p.53 / Chapter III.7 --- Differences between cAMP- and ionophore- dependent C1- current --- p.57 / Chapter III. 8 --- The swelling-induced whole-cell currents --- p.63 / Chapter III.9 --- The swelling-induced current was mainly C1- selective --- p.65 / Chapter III. 10 --- Anion selectivity of the swelling-induced C1- current --- p.68 / Chapter III. 11 --- Inhibition of the swelling-induced C1- conductance by anion channel blockers --- p.68 / Chapter III. 12 --- The role of Ca2+ and cAMP in swelling-induced C1- conductance --- p.74 / Chapter Chapter IV. --- Discussion --- p.79 / Signal transduction mechanism of adrenaline stimulated C1- current in human epididymal cell --- p.79 / Anion secretion in rat epididymal cell The role of Ca2+ and cAMP --- p.83 / Chapter Chapter V. --- Reference --- p.88
6

Adrenergic control and its mechanism of stimulation of electrogenic anion secretion in primary cultures of rat epididymal eipthelialcells

陳浦棠, Chan, Po-tong, Timothy. January 1990 (has links)
published_or_final_version / Physiology / Master / Master of Philosophy

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