<p> This study is one of the first to examine non-targeted effects of radiation in fish
cell lines, with the aim of identifying a reliable reporter system for evaluating radiation
damage in fish. The ability of the fish cell lines to clone was determined as the
clonogenic assay was a major end point used to measure survival. A direct survival
curve was completed for all cell lines that were deemed clonogenic using a cobalt-60 γ-radiation
source. Non-targeted effects of radiation were evaluated by conducting
bystander experiments on all fish cell lines. Delayed Cell Death (DCD) experiments
were completed on the fish cell line that showed evidence of a cell death associated
bystander effect as these phenomena may be linked. Four of the eight cell lines were
found to be clonogenic. The cell line, RTG-2, was found to be the most radiosensitive at
lower doses. All of the clonogenic cell lines, with the exception ofRTG-2 cells,
generally showed increased Plating Efficiency (P.E.) when Irradiated Cell Conditioned
Media (ICCM) was tested on unirradiated autologous cells. ICCM from the clonogenic
and non-clonogenic cells was also tested on the mammalian cell line. This resulted in
increased cell survival, with the exception of the RTS-pBk+ (p<O.OOl), RTS-34st
(p<O.Ol) and ZEB 2J (p<0.05) cell lines. Since RTG-2 showed the most prominent cell
killing bystander effect, DCD experiments were performed on this cell line. DCD was
found in the progeny of irradiated parental cells at all doses tested. Cell kinetics also
showed the generation of possible DCD. The results show that both bystander signal
production and cellular responses vary depending on the cell line and that DCD and
bystander effects are tentatively linked through genomic instability. The RTG-2 cell line may be a suitable model for a reliable reporter system to aid in determining the nontargeted
effects of radiation in fish in the environment. </p> / Thesis / Master of Science (MSc)
| Identifer | oai:union.ndltd.org:mcmaster.ca/oai:macsphere.mcmaster.ca:11375/21665 |
| Date | 01 1900 |
| Creators | O'Neill, Alicia |
| Contributors | Mothersill, C., Biology |
| Source Sets | McMaster University |
| Language | English |
| Detected Language | English |
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