FundaÃÃo de Amparo à Pesquisa do Estado do Cearà / Os procedimentos cirÃrgicos para tratamento das doenÃas dos cÃlons sÃo comuns
na prÃtica mÃdica. As deiscÃncias de anastomoses colÃnicas podem determinar considerÃveis morbidade e mortalidade. A cicatrizaÃÃo destas anastomoses à um
processo complexo, e vÃrios fatores podem modificar sua evoluÃÃo. Os antiinflamatÃrios tÃm interessado aos pesquisadores, pelo potencial de interferir nas fases de inflamaÃÃo e fibroplasia do processo cicatricial. A aroeira-do-sertÃo (Myracrodruon urundeuva Fr. All.) Ã uma planta usada popularmente como antiinflamatÃrio e promotor da cicatrizaÃÃo, em vÃrias afecÃÃes. Neste estudo, o objetivo foi avaliar a aÃÃo do extrato aquoso da aroeira-do-sertÃo a 10%, sob a forma de enemas, na anastomose colÃnica, em ratos. Foram utilizados 48 ratos da
linhagem Wistar, machos, com peso mÃdio 320 g, distribuÃdos em dois grupos, com 24 animais, cada. Todos os animais foram submetidos à laparotomia, com secÃÃo transversa completa do cÃlon descendente, seguida de anastomose colÃnica. Os ratos do grupo A receberam diariamente, no pÃs-operatÃrio, enemas de veÃculo à base de carboximetilcelulose (CMC), atà a data da eutanÃsia. Os animais do grupo B receberam diariamente, no pÃs-operatÃrio, enemas de extrato aquoso de aroeira-do sertÃo a 10% em veÃculo à base de CMC. Nas datas 3, 7, 14 e 21 do experimento, seis ratos de cada grupo foram submetidos à remoÃÃo de segmento colÃnico, incluindo a anastomose, destinado à apreciaÃÃo histolÃgica e anÃlise qualitativa e quantitativa da resposta celular inflamatÃria e cicatricial. A evoluÃÃo morfolÃgica do processo cicatricial foi significantemente menor no grupo aroeira que no grupo controle, no dia 7 do experimento (ρ < 0,05). Na avaliaÃÃo morfomÃtrica, verificou-se menor migraÃÃo neutrofÃlica, nos dias 7 e 21, e mais angiogÃnese e fibrogÃnese, no dia 14, no grupo aroeira (ρ < 0,05). TambÃm houve menor deposiÃÃo do colÃgeno, nos dias 3, 7 e 14 do experimento, no grupo aroeira (ρ < 0,05). Conclui-se, portanto, que a aroeira à eficaz como antiinflamatÃrio, pois diminui a intensidade da resposta inflamatÃria aguda inicial (dia 7), durante a cicatrizaÃÃo das anastomoses colÃnicas. Contudo, apesar de demonstrar aÃÃo angiogÃnica e fibrogÃnica (dia 14), hà retardo na deposiÃÃo de colÃgeno (dias 3, 7 e 14), no grupo aroeira. Mais tardiamente (dia 21), entretanto, a aroeira nÃo causa alteraÃÃes histolÃgicas no processo cicatricial / The surgical procedures for treatment of colonic diseases are common in medical
practice. Colonic anastomoses dehiscences can determine considerable morbidity
and mortality. The healing of these anastomoses is a complex process, and several factors can modify its evolution. Anti-inflammatories have interested researchers, for the potential to interfere on the phases of inflammation and fibroplasia of the healing process. The aroeira-do-sertÃo (Myracrodruon urundeuva Fr. All.) is a plant popularly used as an anti-inflammatory and promoter of healing, in several affections. In this study, the purpose was to evaluate the action of the 10% aqueous extract of aroeirado- sertÃo, under the form of enemas, on colonic anastomosis, in rats. There were utilized 48 Wistar rats, males, with average weight 320 g, distributed in two groups, with 24 animals, each. All animals were subjected to laparotomy, with complete transverse section of the descending colon, followed by colonic anastomosis. The rats on group A received daily, post-operative, enemas of carboxymethylcellulose (CMC) based vehicle, until the date of euthanasia. The animals on group B received daily, post-operative, enemas of 10% aqueous extract of aroeira-do-sertÃo in CMC based vehicle. On dates 3, 7, 14 and 21 of the experiment, six rats in each group were subjected to removal of a colonic segment, including the colonic anastomosis, destined to histological appreciation and qualitative and quantitative analysis of inflammatory and healing cell response. The morphological evolution of the healing process was significantly lower on the group aroeira than on the group control, on day 7 of the experiment (ρ < 0,05). On the morphometric evaluation, it was verified less neutrophilic migration, on days 7 and 21, and more angiogenesis and fibrogenesis, on day 14, on the group aroeira (ρ < 0,05). There was also less deposition of collagen, on days 3, 7 and 14 of the experiment, on the group aroeira (ρ < 0,05). It is concluded, therefore, that aroeira is efficient as an anti-inflammatory, because it diminishes the intensity of the initial (day 7) acute inflammatory response, during the healing of colonic anastomoses. However, in spite of showing angiogenic and fibrogenic action (day 14), there is delay on collagen deposition (days 3, 7 and 14), on the group aroeira. Later on (day 21), however, aroeira does not cause histological alterations on the healing process
| Identifer | oai:union.ndltd.org:IBICT/oai:www.teses.ufc.br:348 |
| Date | 26 August 2005 |
| Creators | Annya Costa AraÃjo de MacÃdo Goes |
| Contributors | Lusmar Veras Rodrigues, Jose Alberto Dias Leite, Marcus Vinicius Henriques Brito |
| Publisher | Universidade Federal do CearÃ, Programa de PÃs-GraduaÃÃo em Cirurgia, UFC, BR |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFC, instname:Universidade Federal do Ceará, instacron:UFC |
| Rights | info:eu-repo/semantics/openAccess |
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