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guarizafilho_o_dr_arafo.pdf: 1240531 bytes, checksum: c7e1cb0f17d9a4a706f95d4f47284151 (MD5) / O objetivo deste trabalho foi elucidar os mecanismos do ácido fólico em prevenir o aparecimento de fenda palatina teratogenicamente induzida pela dexamatasona em ratos. Para tanto, foram usadas um total de 13 ratas do tipo Holtzman. G1) 3 ratas receberam 3mg/Kg Dex. Intramuscular (i.m.) do 14o ao 17o dia de prenhez. G2) 4 ratas receberam 3mg/Kg Dexa (i.m.) do 14o ao 17o dia de prenhez, e uma dose de 5mg/kg de ácido fólico (via oral) do dia 14o até 17o de prenhez. G3) 3 ratas receberam 3mg/Kg Dexa (i.m.) do 14o ao 17o dia de gravidez e uma dose de 5mg/kg de ácido fólico (via oral) do início até o final da prenhez. G4) 3 ratas receberam 3mg/Kg Dex (i.m.) do 14o ao 17o dia de prenhez e uma dose de 5mg/kg de ácido fólico (via oral) desde 5 dias antes da concepção até o final da gravidez. As cabeças dos fetos foram removidas no 19o dia de prenhez e estudadas. Foi utilizado o teste não paramétrico de Kruskal-Wallis. O resultados mostraram o seguinte: G1) dos 27 fetos, (96,30%) apresentaram fenda total, e apenas 1 apresentou fenda parcial. G2) Dos 23 fetos apenas (8,7%) com fenda total, (43,35%) com ausência de fenda e (47,80%) apresentaram fenda parcial. G3) Observou-se 88,24% apresentaram fenda incompleta, 5,88% apresentaram fenda completa e 5,88% ausência de fenda. G4) dos 31 animais, (54,4%) apresentaram fenda incompleta, e 32,26% apresentaram fenda completa e (12,9%) sem fenda. / The aim of the present study is to determine whether (FA) prevented cortisone-induced cleft palate (CP) in the Holtzman mouse. The animals were divided in 4 groups: G1- 3 pregnant mice had 3mg/Kg of Dex intramuscularly administrated (IA) from the 14th to 17th day of gestation; G2- 4 pregnant mice had 3mg/Kg of Dex IA from the 14th to 17th day of gestation, with a daily dose (dd) of 5mg/kg FA oral administrated (OA) from 14th to 17th day of gestation; G3- 3 pregnant mice had 3mg/Kg of Dex IA from the 14th to 17th day of gestation, with a (dd) of 5mg/kg FA OA from day 0 until 17th day of gestation; G4- 3 pregnant mice had 3mg/Kg of Dex IA from 14th to 17th day of gestation, with a (dd) of 5mg/kg FA OA 5 days before the fertilization until the 17th day of gestation. The fetuses were examined on 19th day of gestation. The data was analyzed by not paired Kruskal-Wallis test, and showed statistical difference among average score values. The results demonstrated that (96,3%) (G1) had total CP, and only 1 partial CP. In the (G2), (8,7%) showed total CP, (47,8%) partial CP and (43,35%) showed no CP. The (G3) showed (5,88%) had total CP, (88,24%) partial CP and (5,88%) had no CP. In the (G4), (54,4%) showed partial CP and (32,26%) total CP. Only (12,9%) had no CP. These results indicated that FA reduces the incidence of Dex-induced cleft palate independently on the period of the administration.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.unesp.br:11449/104502 |
| Date | 28 August 2006 |
| Creators | Guariza Filho, Odilon [UNESP] |
| Contributors | Universidade Estadual Paulista (UNESP), Ramalho, Lizete Toledo de Oliveira [UNESP] |
| Publisher | Universidade Estadual Paulista (UNESP) |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | 156 f. : il. |
| Source | Aleph, reponame:Repositório Institucional da UNESP, instname:Universidade Estadual Paulista, instacron:UNESP |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -1, -1 |
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