Made available in DSpace on 2016-04-28T20:17:26Z (GMT). No. of bitstreams: 1
2006-Juan Rojas Pereira.pdf: 760746 bytes, checksum: 1798bbe7196bc85bf9d9b42cb797bfc4 (MD5)
Previous issue date: 2006-03-17 / The aim of this work was to verify the isolation of Cryptococcus neoformans from bird
droppings at some petshops trading in Rio de Janeiro borough-RJ, as well as to perform
biochemical serogruping and to evaluate the virulence in vitro . 1268 samples from several
birds at 25 commercial establishments distributed on 16 neighbourhoods in Rio de Janeiro
Borough-RJ were collected.The biochemical serogrouping was carried out in CGB culture
medium plates while protease and phospholipase production indicating virulence factors
through specific media containing bovine soralbumin and yolk were performed in vitro
respectively. Both virulence and serogrouping assays for 64 strains were carried out. From the
total of samples, 85 (6,7%) were considered positive. Positivity feature was verified for birds,
as well as Melopsittacus undulatus birds droppings, Serinus canaria and other sort of birds
showed a greater member of seedlings. According to serogrouping, from the total of 56
samples, 54 were classified
as serogrouping AD (Cryptococcus neoformans var. neoformans) and just two of them to
serogrouping BC (Cryptococcus neoformans var gattii) possibly.All the strains showed to be
protease and phospholipase producers and most as hard ones. Of the isolated 56 all were fit in
the biotypes "Killer" I and II (var neoformans). Talking into account the possibility of high
level infection for human and animal helth, the presence of this ethiological agent on such
kind of establishments, it might be a great concern to sanitary surveillance policy for
controllling the infection, furthermore. / O objetivo do presente trabalho foi verificar o isolamento de Cryptococcus neoformans em
excretas de aves comercializadas em pet-shops localizados no Munic?pio do Rio de Janeiro
RJ, realizar a sorogrupagem bioqu?mica e avaliar a virul?ncia in vitro . Foram colhidas
1268 amostras de diversas aves em 25 estabelecimentos distribu?dos em 16 bairros do referido
munic?pio. A sorogrupagem bioqu?mica foi realizada em meio CGB, enquanto a
determina??o da produ??o de protease e de fosfolipase como fatores de virul?ncia, foi
realizada in vitro pelo emprego de meios espec?ficos contendo respectivamente
soroalbumina bovina e gema de ovo. Do total de amostras avi?rias coletadas, 85 (6,70%)
mostraram-se positivas. A positividade foi verificada isoladamente para as aves, sendo que as
excretas de Melopsittacus undulatus, Serinus canaria e outras aves de maior procura
destacaram-se pelo maior n?mero de isolados. A sorogrupagem realizada para 56 cepas
revelou que 54 amostras pertencem ao sorogrupo AD (Cryptococcus neoformans variedade
neoformans) e duas, possivelmente ao sorogrupo BC (C. neoformans var gattii). Todas as
cepas mostraram-se produtoras de protease e de fosfolipase e a maioria revelou-se forte
produtora destas enzimas. Dos 56 isolados todos se encaixaram nos bi?tipos Killer I e II
(var neoformans). A presen?a deste agente em tais estabelecimentos comerciais deve ser
motivo de preocupa??o para as autoridades sanit?rias, considerando-se a possibilidade de
infec??o para o homem e para outros animais ali comercializados.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:tede/862 |
| Date | 17 March 2006 |
| Creators | Pereira, Juan Rojas |
| Contributors | Baroni, Francisco de Assis |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Microbiologia Veterin?ria, UFRRJ, Brasil, Microbiologia Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
Page generated in 0.0027 seconds