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Sustained off-resonance irradiation collision-induced dissociation of peptide ions in a fourier transform ion-cyclotron-resonance mass spectrometer.

Duan Lifang. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (leaves 83-87). / Abstracts in English and Chinese. / Title Page --- p.i / Abstract (English) --- p.ii / Abstract (Chinese) --- p.iii / Acknowledgements --- p.iv / Table of Contents --- p.v / List of Tables --- p.vii / List of Figures --- p.viii / Symbols and Abbreviations --- p.x / Chapter CHAPTER 1 --- INTRODUCTION / Chapter 1.1 --- Mass spectrometry in biochemistry --- p.1 / Chapter 1.2 --- Electrospray ionization (ESI) --- p.2 / Chapter 1.3 --- Fourier transforms ion cyclotron resonance mass spectrometry (FTICR-MS) --- p.4 / Chapter 1.3.1 --- Ion motions --- p.5 / Chapter 1.3.2 --- Mass calibration --- p.7 / Chapter 1.3.3 --- Tandem mass spectrometry --- p.11 / Chapter 1.4 --- Sustained off-resonance irradiation collision-induced dissociation (SORI-CID) of peptide ions --- p.13 / Chapter 1.5 --- Outline of present work --- p.18 / Chapter CHAPTER 2 --- INSTRUMENTATION AND EXPERIMENTAL / Chapter 2.1 --- Fourier-trans form ion cyclotron resonance mass spectrometer --- p.19 / Chapter 2.1.1 --- Vacuum system --- p.19 / Chapter 2.1.2 --- Nanospray source --- p.23 / Chapter 2.1.3 --- Ion transfer system --- p.26 / Chapter 2.1.4 --- Infinity cell --- p.28 / Chapter 2.1.5 --- Data acquisition system --- p.29 / Chapter 2.2 --- Experimental --- p.29 / Chapter 2.2.1 --- Simple acquisition pulse program --- p.30 / Chapter 2.2.2 --- Tandem mass spectrometry (SORI-CID MS/MS) --- p.32 / Chapter CHAPTER 3 --- DISSOCIATION OF MODEL PEPTIDE IONS UNDER SORI-CID CONDITIONS / Chapter 3.1 --- Introduction --- p.35 / Chapter 3.2 --- Experimental --- p.37 / Chapter 3.3 --- Results and discussion --- p.41 / Chapter 3.3.1 --- "SORI-CID of XG3WG3X (where X 二 R, K and N)" --- p.41 / Chapter 3.3.2 --- SORI-CID of RGnWGnR (where n=l -5) --- p.46 / Chapter 3.3.3 --- SORI-CID of RG3XG3R (where X = D and E) --- p.48 / Chapter 3.4.4 --- SORI-CID of sequence isomers of RG5WG5R --- p.51 / Chapter 3.4 --- Conclusions --- p.56 / Chapter CHAPTER 4 --- EXACT MASS MEASUREMENTS OF THE PRODUCT IONS DERIVED FROM SORI-CID FTICR-MS / Chapter 4.1 --- Introduction --- p.57 / Chapter 4.2 --- Experimental --- p.60 / Chapter 4.3 --- Results and discussion --- p.61 / Chapter 4.3.1 --- Origin(s) of the mass measurement error --- p.63 / Chapter 4.3.2 --- Pressure effect on the cyclotron frequencies of trapped ions --- p.67 / Chapter 4.3.3 --- Modified internal lock-mass calibration method --- p.70 / Chapter 4.4 --- Conclusions --- p.79 / Chapter CHAPTER 5 --- CONCLUSIONS --- p.81 / REFERENCES --- p.83 / APPENDIX I The Proton affinity (PA) and the structural formulae of 20 α-amino acids --- p.88 / APPENDIX II Schematics of the proposed mechanisms of peptide dissociation under low-energy CID conditions --- p.89 / APPENDIX III Pulse programs for simple MS and MS/MS experiments --- p.92 / APPENDIX IV Schematics of the proposed mechanisms for the formation of y8+ ions from the singly protonated arginine- and lysine-containing peptides --- p.99

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324347
Date January 2003
ContributorsDuan, Lifang., Chinese University of Hong Kong Graduate School. Division of Chemistry.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, x, 100 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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