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Previous issue date: 2011-08-03 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The suprachiasmatic nucleus (SCN) of the anterior hypothalamus, together with the
intergeniculate leaflet (IGL) of the thalamus are considered the central components of the
circadian timing system (CTS) of mammals. This system is responsible for the generation and
regulation of circadian rhythms by establishing a temporal organization of physiological
processes and behaviors. The neuronal specific nuclear protein (NeuN) has been widely used
as a neuronal marker in several studies. Since glial fibrillary acidic protein (GFAP) is a
component of intermediate filaments found in the cytoplasm of astrocytes and is commonly
used as a specific marker for these cells. This study aims to identify, in the marmoset, the
NeuN immunoreactive neurons and glial cells immunoreactive to GFAP, as well as map the
major route of photic synchronization of the STC, retinohypothalamic tract (RHT), and
identify the indirect pathway to the SCN and pregeniculate nucleus (PGN) - structure
homologous to IGL rodents, using immunohistochemical and cytoarchitectonic techniques.
Observed in SCN the presence of neurons immunoreactive to NeuN and terminals
immunoreactive subunit b of cholera toxin (CTb), neuropeptide Y (NPY) and serotonin (5-
HT). In the PGN noted the presence of the NeuN and NPY immunoreactive neurons and the
immunoreactive terminals CTb and 5-HT. Astrocytes are present throughout the extent of the
SCN and the PGN this New World primate / O n?cleo supraquiasm?tico (NSQ) do hipot?lamo anterior, juntamente com o folheto
intergeniculado (FIG) do t?lamo s?o considerados os componentes centrais do sistema de
temporiza??o circadiana (STC) de mam?feros. Tal sistema ? respons?vel pela gera??o e
regula??o dos ritmos circadianos estabelecendo uma organiza??o temporal dos processos
fisiol?gicos e comportamentos. A prote?na nuclear neuronal espec?fica (NeuN) tem sido
amplamente utilizada como um marcador neuronal em diversos estudos. J? a prote?na ac?dica
fibrilar glial (GFAP) ? um componente dos filamentos intermedi?rios encontrada no
citoplasma dos astr?citos e ? comumente usada como um marcador espec?fico para essas
c?lulas. Este trabalho tem como objetivo identificar, no sagui, neur?nios imunorreativos a
NeuN e c?lulas gliais imunorreativas a GFAP, bem como mapear a principal via de
sincroniza??o f?tica do STC, o trato retinohipotal?mico (TRH), e identificar as vias indiretas
para o NSQ e n?cleo pr?-geniculado (NPG) estrutura hom?loga ao FIG dos roedores,
utilizando t?cnicas citoarquitet?nica e imunoistoqu?mica. Observamos no NSQ a presen?a de
neur?nios imunorreativos a NeuN, bem como terminais imunorreativos a subunidade b da
toxina col?rica (CTb), a neuropept?do Y (NPY) e a serotonina (5-HT). J? no NPG notamos a
presen?a de neur?nios imunorreativos a NeuN e a NPY e terminais imunorreativos a CTb e a
5-HT. Os astr?citos est?o presentes em toda a extens?o do NSQ e do NPG deste primata do
Novo Mundo
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/17322 |
| Date | 03 August 2011 |
| Creators | Nascimento, Rayane Bartira Silva do |
| Contributors | CPF:70317232487, http://lattes.cnpq.br/2378505945149958, Pinato, Luciana, CPF:06964593888, http://lattes.cnpq.br/8372363591179624, Costa, Miriam Stela Maris de Oliveira, CPF:05014077404, http://lattes.cnpq.br/8424747565185682, Cavalcante, Jeferson de Souza |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Psicobiologia, UFRN, BR, Estudos de Comportamento; Psicologia Fisiol?gica |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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