In the tuberculosis patients, Mycobacterium tuberculosis can stimulate production of
hydrogen peroxide in the host as a result of immune response. The H2O2 accumulate
in pulmonary cells, causing oxidative stress that could lead to the cancer. We select
TB patients for this study which investigates the effects of quercetin as there is an
increased incidence of latent TB among the migrant population in the past few years
and TB can increase the risk of cancer.
Sperm and lymphocytes were treated with DNA damage inducers and quercetin
(10µM, 25µM and 100µM), the responses evaluated using the Comet and
micronucleus techniques. The gene expressions of COX1, COX2, P53 and Bcl-2 and
catalase protein expression were investigated using the qPCR and Western blot
techniques.
The results showed that a substantial reduction of DNA damage in lymphocytes from
TB patients and sperm from healthy donors from * P ≤ 0.0283 to *** P≤0.001in the
Comet assay. In the MNi assay, the effect of quercetin in lymphocytes was more
significant in reduce DNA damage, whereas the DNA damage induced by a food
mutagen was significant, from *p 0.0405 to ***p 0.001. The qPCR showed
significance down-regulation of COX1 and Bcl-2 gene expression, rated between *p 0.045 and **p 0.0074. However, the catalase protein was up-regulated by the nano
form of quercetin when using lymphocytes from TB patients and showed significant
changes at *p 0.0236.
In conclusion, the nano form was found to be more efficient at the reduction of DNA
damage in the Comet and micronucleus assays. Also, it down-regulated COX1 and
Bcl-2 and up-regulated the catalase proteins indicating a possible role for quercetin,
in genoprotection to TB through its enzyme modulating effect. / Libyan Embassy
| Identifer | oai:union.ndltd.org:BRADFORD/oai:bradscholars.brad.ac.uk:10454/18335 |
| Date | January 2019 |
| Creators | Abdulmwli, Mhamoued A.A. |
| Contributors | Anderson, Diana, Gopalan, Rajendran C. |
| Publisher | University of Bradford, School of Chemistry and Bioscience |
| Source Sets | Bradford Scholars |
| Language | English |
| Detected Language | English |
| Type | Thesis, doctoral, PhD |
| Rights | <a rel="license" href="http://creativecommons.org/licenses/by-nc-nd/3.0/"><img alt="Creative Commons License" style="border-width:0" src="http://i.creativecommons.org/l/by-nc-nd/3.0/88x31.png" /></a><br />The University of Bradford theses are licenced under a <a rel="license" href="http://creativecommons.org/licenses/by-nc-nd/3.0/">Creative Commons Licence</a>. |
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