The hypothalamic arcuate nucleus (Arc) is a central unit that controls the appetite through
the integration of metabolic, hormonal, and neuronal afferent inputs. Agouti-related protein (AgRP),
proopiomelanocortin (POMC), and dopaminergic neurons in the Arc differentially regulate feeding
behaviors in response to hunger, satiety, and appetite, respectively. At the time of writing, the anatomical
and electrophysiological characterization of these three neurons has not yet been intensively
explored. Here, we interrogated the overall characterization of AgRP, POMC, and dopaminergic
neurons using genetic mouse models, immunohistochemistry, and whole-cell patch recordings.
We identified the distinct geographical location and intrinsic properties of each neuron in the Arc
with the transgenic lines labelled with cell-specific reporter proteins. Moreover, AgRP, POMC, and
dopaminergic neurons had different firing activities to ghrelin and leptin treatments. Ghrelin led to
the increased firing rate of dopaminergic and AgRP neurons, and the decreased firing rate of POMC.
In sharp contrast, leptin resulted in the decreased firing rate of AgRP neurons and the increased
firing rate of POMC neurons, while it did not change the firing rate of dopaminergic neurons in Arc.
These findings demonstrate the anatomical and physiological uniqueness of three hypothalamic Arc
neurons to appetite control.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:89137 |
| Date | 18 January 2024 |
| Creators | Na, Junewoo, Park, Byong Seo, Jang, Doohyeong, Kim, Donggue, Tu, Thai Hien, Ryu, Youngjae, Ha, Chang Man, Koch, Marco, Yang, Sungchil, Kim, Jae Geun, Yang, Sunggu |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 2609 |
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