Field pea (Pisum sativum L.) is highly susceptible to ascochyta blight, primarily caused by the pathogen Mycosphaerella pinodes (Berk. & Blox.) Vestergr. Grasspea (Lathyrus sativus L.) has been reported to possess a moderate level of resistance to ascochyta blight caused by M. pinodes. The work reported in this thesis aimed to develop the various techniques that would be required to transfer ascochyta blight resistance from grasspea into field pea via somatic hybridisation. This thesis also assesses the feasibility of achieving this goal. Field pea shoot cultures were established on hormone-free MS medium, and a protoplast isolation protocol developed for both species. Grasspea shoot cultures were established on both RL and SSB8 medium. Friable grasspea callus was achieved on media supplemented with 2,4-D in the range 4.523 µM, whereas kinetin tested at any concentration did not appear to influence callus growth. A suspension culture of grasspea was developed for the first time, in B5 medium supplemented with 4.5 µM 2,4-D and 0.5 µM kinetin. Grasspea protoplasts were isolated from both in vitro seedlings and suspension cultures. Protocols for hybrid shoot culture on KM8p medium were developed via organogenesis and somatic embryogenesis.
| Identifer | oai:union.ndltd.org:ADTP/279016 |
| Creators | McCutchan, Jennifer Susan |
| Source Sets | Australiasian Digital Theses Program |
| Detected Language | English |
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