In the past study, we used a human gastric stem cell clone, KMU-GI2, isolated from endoscopically biopsied gastric mucosa. As we maintained this KMU-GI2 cell clone, we also found a subclone with spontaneous transformation, named KMU-CSN. We found this KMU-CSN cell line showed homogenous epithelial morphology, cell pile-up appearance due to contact insensitivity, enhanced anchorage independence (from 1.7% of KMU-GI2 to 22% of KMU-CSN) and cell immortalization (CPDL >100). The high tumorigenic potential of this KMU-CS12 cell line has been endorsed by the finding of the tumor formation in nude mice. By spectral karyotyping and SNP GeneChip® Mapping 500K Assay, we found chromosomal abnormalities in chromosome 12, which were derived from duplication of 7p15.1~15.3 and 7p22.1~22.3. In normal cells, HOXA genes were found to localize in chromosome 7. In this KMU-CS12 cell line, an increased expression of HOXA4¡B5¡B7¡B9¡B13 genes was also shown, as compared with KMU-CSN. With these findings, we hypothesized that HOXA genes may play a role in gastric carcinogenesis. Therefore, we can check the prevalence of HOXA genes abnormalities in human gastric cancer patients.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0623109-181128 |
| Date | 23 June 2009 |
| Creators | Huang, Jian-yuan |
| Contributors | JAW-YUAN WANG, Deng-Chyang Wu, Angela Chen |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0623109-181128 |
| Rights | withheld, Copyright information available at source archive |
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