Infection of fully expanded leaves of tobacco with INF1 causes the appearance of HR lesions within 12 h and progressive to all infection sites after 48 h treatment. Among the POD isozymes, the increase of cationic PODs and anionic PODs is correlated with the rise of lignin contents in INF1-treated leaves, especially cationic PODs (pI 9.5, pI 8.7, pI 8.3, pI 7.8, pI 7.4). It was suggested that the induction of POD activity resulted in part of H2O2 reduction. The increase of cationic (pI 9.5) and anionic (pI 4.4) POD transcripts was correlated with the increased cationic and anionic PODs activity in INF1-treated leaves. Therefore, the increased POD activity is due to the de novo synthesis of the cationic (pI 9.5) and anionic (pI 4.4) PODs in INF1-treated leaves. The increase in cationic pI 9.6 laccase transcript was also correlated with the increased cationic laccase activity in INF1-treated leaves. Our results suggest that laccase might play a major role on lignin biosynthesis at the early stage (6 h), and as the inoculation time was prolonged, peroxidases (especially cationic POD) and laccases will work together on lignin biosynthesis.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0605104-183446 |
| Date | 05 June 2004 |
| Creators | Wang, Li-Ting |
| Contributors | Ching-Mei Hsu, none, Zin-Huang Liu |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0605104-183446 |
| Rights | off_campus_withheld, Copyright information available at source archive |
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