Harpinpss, a pathogenic protein, encoded by hrpZ in the hrp gene cluster from Pswudomonas syringae pv. syringae, can induce the hypersensitive response in tobacco (Nicotiana tabacum L. cv. Xanthi). The lesion area on the tobacco leaves was visible 6 h after inoculation with harpin, and was evident 12 h after inoculation. The lignin content in harpin-treated tobacco leaves was about 2.5-fold as compared with the controls 24 h after inoculation.
There were six isozymes of POD (pI 9.5, pI 8.7, pI 5.3, pI 4.4, pI 3.7, and pI 3.5) and seven isozymes of laccase (pI 9.4, pI 8.6, pI 7.8, pI 5.4, pI 4.5, pI 3.8, and pI 3.6) identified by isoelectric point in extracts of harpin-inoculated tobacco leaves. POD isozymes (pI 4.4, pI 5.3 and pI 8.7) and laccase isozyme (pI 7.8) only appeared in harpin-inoculated tissues. The increased POD isozymes (pI 4.4, pI 8.7, pI 9.5) are correlated with the rise of transcripts of these enzymes confirmed by the method of reverse transcriptase-polymerase chain reaction (RT-PCR).
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0620103-161326 |
| Date | 20 June 2003 |
| Creators | Jan, Jen-Ting |
| Contributors | Zin-Huang Liu, Hao-Jen Huang, Ching-Mei Hsu |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0620103-161326 |
| Rights | unrestricted, Copyright information available at source archive |
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