The World Health Organization (WHO) has estimated that 10.4 million new cases of tuberculosis (TB) occurred worldwide in 2015, with a steady increase in resistance cases. Considering TB control difficulties, it is necessary to discover new drugs, especially for resistant strains, and it is believed that medicinal plants, through their essential oils (EOs), are one of the alternatives for bioprospecting active substances, such as the EO extracted from the bark of Cinnamomum zeylanicum (EOCzey), popularly known as cinnamon, and trans-cinnamaldehyde (TCin), its compound, which has several therapeutic properties, such as antimicrobial, anti-inflammatory, and hypoglycemic action, among others. In this context, the present study evaluated the antimycobacterial action of EOCzey and TCin on sensitive and resistant clinical isolates (CI) of M. tuberculosis, as well as the effects of the associations between EOCzey and TCin and the drugs rifampicin (RIF) and isoniazid (INH) on the standard M. tuberculosis H37Rv strain. The Resazurin Microtiter Assay (REMA) technique was used to determine the minimum inhibitory concentration (MIC) of the compounds tested on nine M. tuberculosis CIs. The effects of the EOCzey / RIF, EOCzey / INH, TCin / RIF and TCin / INH associations on the standard M. tuberculosis H37Rv strain were evaluated according to the checkerboard method, determining the Fraction Inhibitory Concentration Index (FICi), in which the synergistic effect was observed with FICi values ≤ 0.5, additive effect with FICi >0.5 and ≤ 1 and antagonism with FICi >1.0. It was observed that EOCzey and TCin were able to inhibit the growth of all nine sensitive and resistant CIs with MIC values ranging from 9.75 μg / mL to 39 μg / mL. All four associations tested resulted in synergistic and additive effects, with no antagonistic effect. Both EOCzey / RIF and EOCzey / INH associations showed FICi values of 0.375 and 0.5, whereas the TCin / RIF and TCin / INH associations showed FICi values of 0.31, 0.375 and 0.5. / A OrganizaÃÃo Mundial de SaÃde (OMS) estimou a ocorrÃncia de 10,4 milhÃes casos novos de tuberculose (TB) no mundo em 2015 com um aumento contÃnuo dos casos de resistÃncia. Com a difilcudade de controle TB, existe a necessidade da descoberta de novos fÃrmacos, especialmente sobre cepas resistentes e acredita-se que as plantas medicinais, atravÃs dos Ãleos essenciais (OEs), sejam uma das alternativas para bioprospecÃÃo de substÃncias ativas, como o OE extraÃdo da casca de Cinnamomum zeylanicum (OECzey), popularmente conhecida com canela, e o trans-cinamaldeÃdo (TCin), seu composto, que possuem diversas propriedades terapÃuticas, como aÃÃo antimicrobiana, anti-inflamatÃria, hipoglicemiante, entre outras. Nesse contexto, o presente estudo avaliou a aÃÃo antimicobacteriana do OECzey e do TCin sobre isolados clÃnicos (ICs) de M. tuberculosis, sensÃveis e resistentes, como tambÃm os efeitos das associaÃÃes entre o OECzey e TCin com os fÃrmacos rifampicina (RIF) e isoniazida (INH) sob a cepa padrÃo M. tuberculosis H37Rv. Utilizou-se a tÃcnica Resazurin Microtiter Assay (REMA) para determinaÃÃo da concentraÃÃo inibitÃria mÃnima (CIM) dos compostos testados sobre nove ICs de M. tuberculosis. Os efeitos das associaÃÃes OECzey/RIF, OECzey/INH, TCin/RIF e TCin/INH sobre a cepa padrÃo M. tuberculosis H37Rv foram avaliadas de acordo com mÃtodo Checkerboard, determinando-se o Ãndice de ConcentraÃÃo InibitÃria Fracionada (IFIC), no qual efeito sinÃrgico foi observador com valores de IFIC ≤ 0,5, efeito aditivo com IFIC > 0,5 e ≤ 1 e antagonismo com IFIC > 1,0. Observou-se que OECzey e o TCin foram capazes de inibir o crescimento de todos os nove ICs, sensÃveis e resistentes, com valores de CIM variando entre 9,75 μg/mL a 39 μg/mL. Todas as quatro associaÃÃes testadas resultaram em efeito sinÃrgico e aditivo, com ausÃncia efeito antagÃnico. Ambas associaÃÃes OECzey/RIF e OECzey/INH apresentaram valores de ICIF de 0,375 e 0,5, enquanto que nas associaÃÃes TCin/RIF e TCin/INH os valores de IFIC foram 0,31, 0,375 e 0,5.
| Identifer | oai:union.ndltd.org:IBICT/oai:www.teses.ufc.br:12095 |
| Date | 23 February 2017 |
| Creators | Aquiles Paulino Peres Mota |
| Contributors | Cristiane Cunha Frota, Nadia Accioly Pinto Nogueira, Edson Holanda Teixeira, Ãrika Helena Salles de Brito |
| Publisher | Universidade Federal do CearÃ, Programa de PÃs-GraduaÃÃo em Patologia, UFC, BR |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFC, instname:Universidade Federal do Ceará, instacron:UFC |
| Rights | info:eu-repo/semantics/openAccess |
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