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Previous issue date: 2015-09-28 / In this study, we evaluated the antinociceptive, antiinflammatory and antioxidant effects of lavender essential oil. The antioxidant activity was tested in vitro and used as screening for subsequent experiments. In animals, the effect of lavender essential oil was compared to those displayed by known antiinflammatory and analgesic drugs (dexamethasone, tramadol and indomethacine). Attempts have been made in order to suggest a probable mechanism of action. Considering the pharmacological potential of the lavender essential oil, we have also investigated its possible toxic effects. We demonstrated that the dose of 0,6 g/kg is well tolerated orally. This dose was considered safe and was used in subsequent experiments. The antiinflamatory activity was tested using two models of acute inflammation: the pleurisy induced by carrageenan and the ear edema induced by croton oil. The inflammatory response evoked by carrageenan and by croton oil were reduced by the pre-treatment of animals with lavender oil. On pleurisy, the drug used as positive control, dexamethasone, was more efficacious. However, in the ear swelling, the antiedematogenic effect of the oil was similar to the obtained for dexamethasone.The antinociceptive activity was tested using the model of pain induced by formalin injection into the hind paw of rats. In this model, lavender oil consistently inhibited nociception and presented a similar effect to tramadol. This finding suggests that the mechanism involved in the antiinflammatory effect of lavender may be related to interference in the system of intracellular second messenger phospholipase C/inositol phosphate. In conclusion, the results of this study reveal remarkable analgesic and antiinflammatory activities for the lavender essential oil. Furthermore, the effectiveness of the oil associated with the low toxicity support the interest for the application of lavender essential oil in aromatherapy, and demonstrated its important therapeutic potential. / Neste estudo, foram avaliadas as atividades antinociceptiva, antiiflamat?ria e antioxidante do ?leo essencial de lavanda. A atividade antioxidante foi testada in vitro e serviu como screening para os experimentos posteriores. Em animais, o efeito do ?leo essencial de lavanda foi comparado com drogas analg?sicas e anti-inflamat?rias conhecidas (dexametasona, tramadol e indometacina, respectivamente). Isto foi feito na tentativa de sugerir um prov?vel mecanismo de a??o. Considerando o potencial terap?utico do ?leo essencial de lavanda foi realizado tamb?m um estudo de toxicidade aguda, no qual foi demonstrado que o ?leo essencial de lavanda na dose de 600 mg/kg ? bem tolerado oralmente. Esta dose foi considerada segura e utilizada nos experimentos posteriores. A atividade anti-inflamat?ria foi testada atrav?s de dois modelos de inflama??o aguda: pleurisia induzida por carragenina e edema de orelha induzido pelo ?leo de cr?ton. As respostas inflamat?rias produzidas pela carragenina e pelo ?leo de cr?ton foram significativamente reduzidas ap?s o tratamento com ?leo de lavanda.Enquanto na pleurisia a droga usada como controle, dexametasona, foi mais eficaz, no edema de orelha o efeito antiedematog?nico do ?leo foi similar ao observado para a dexametasona. A atividade antinociceptiva foi testada utilizando o modelo de dor induzida por formalina injetada na pata de ratos. Neste modelo, o ?leo de lavanda inibiu a nocicep??o espont?nea provocada pela formalina e apresentou efeito similar ao tramadol. Esses resultados sugerem que o mecanismo envolvido no efeito anti-inflamat?rio da lavanda pode estar relacionado a um antagonismo de receptores acoplados a prote?na G ou interfer?ncia no sistema de segundo mensageiros intracelulares fosfolipase C/fosfato de inositol. Em conclus?o, os resultados deste estudo revelam atividades anti-inflamat?rias e antinociceptivas do ?leo essencial de lavanda. Al?m disso, essas atividades paralelas ? baixa toxicidade encontrada tamb?m suportam o interesse para aplica??o do ?leo de lavanda na aromaterapia e demostram seu importante potencial terap?utico.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/6369 |
| Date | 28 September 2009 |
| Creators | Silva, Gabriela Lucas da |
| Contributors | Oliveira, Jarbas Rodrigues de |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Biologia Celular e Molecular, PUCRS, Brasil, Faculdade de Bioci?ncias |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 8198246930096637360, 600, 600, 600, 36528317262667714, -1634559385931244697 |
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