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Previous issue date: 2014-03-12 / Introduction: Dental pulp is composed of loose connective tissue, which reacts when facing a pathogenic agent, and the inflammation is the first response. DGK? is one of the key enzymes involved on inflammations cellular events. It participates on the neutrophils recruiting to the injury site and on the regulation of superoxide production. The aim of this study was to evaluate the localization of the DGK? in healthy and inflamed/infected dental pulp of rats.
Methods: Were used eighteen (18) male Wistar rats. The animals were divided into three groups of six rats each. In Group 1 (control group), no cavity opening was performed. In Groups 2 and 3 dental pulp of the left first molars was exposed to oral environment for 24hours and 7 days respectively. Euthanasia was performed after the experimental periods and the jaws were dissected for histological evaluation and immunodetection of DGK? enzyme.
Results: Histological analysis of the dental pulp showed that tissue exposure led to inflammatory and degenerative events, which varied in their extension according to the experimental period of time. In groups 2 and 3, DGK? immunolabeling was observed in neutrophils and abscessed areas, being detected on a greater amplitude of pulp tissue in the 24 hour period.
Conclusions: DGK? is expressed on the initial stages of pulp inflammation, although there is no relation with its extent. The understanding of the DGK? role in the pathways of pulp inflammation can help the development of new therapeutic strategies to promote pulp repair. / Introdu??o: A polpa dent?ria ? formada por um tecido conjuntivo frouxo, que reage frente a um agente agressor patog?nico, sendo a inflama??o uma de suas manifesta??es iniciais. A DGK? ? uma das enzimas-chave envolvida em eventos celulares da inflama??o. A mesma participa do recrutamento de neutr?filos para o local da inj?ria e da regula??o da produ??o de super?xido. O objetivo deste estudo foi avaliar, em ratos, a localiza??o da DGK? em polpas saud?veis e em polpas inflamadas/infectadas.
Metodologia: Foram utilizados dezoito (18) ratos machos Wistar. Os animais foram divididos em tr?s grupos de seis animais cada. No grupo 1 (grupo controle), n?o foi realizado nenhum procedimento. Nos grupos 2 e 3, a polpa dent?ria dos primeiros molares do lado esquerdo foi exposta ao meio bucal por 24horas e por 7 dias, respectivamente. A eutan?sia foi realizada ap?s os per?odos experimentais, e as mand?bulas foram dissecadas para an?lise histol?gica e imunolocaliza??o da enzima DGK?.
Resultados: A avalia??o histol?gica da polpa dent?ria mostrou que a exposi??o tecidual levou a eventos inflamat?rios e degenerativos, que variaram de acordo com o per?odo de tempo. Nos grupos 2 e 3, houve marca??o da DGK? em neutr?filos e ?reas pr?ximas a abscessos, sendo detectada em uma maior amplitude no per?odo de 24 horas.
Conclus?es: A DGK? ? expressa em est?gios iniciais da inflama??o, n?o sendo relacionada com a extens?o da inflama??o pulpar. O entendimento do papel da DGK? na cascata de eventos da inflama??o pulpar pode trazer informa??es para o desenvolvimento de novas estrat?gias terap?uticas que promovam o reparo pulpar.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/1257 |
| Date | 12 March 2014 |
| Creators | Fagundes, Gabriela Xavier |
| Contributors | Figueiredo, Jos? Antonio Poli de |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Odontologia, PUCRS, BR, Faculdade de Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 8096554818733665164, 500, 600, 4673435736271820140 |
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