Bone transplantation is regarded as the preferred therapy to treat a variety of bone defects.
Autologous bone tissue is often lacking at the source, and the mesenchymal stem cells (MSCs)
responsible for bone repair mechanisms are extracted by invasive procedures. This study explores
the potential of autologous mesenchymal stem cells derived from the hair follicle outer root sheath
(MSCORS). We demonstrated that MSCORS have a remarkable capacity to differentiate in vitro
towards the osteogenic lineage. Indeed, when combined with a novel gelatin-based hydrogel called
Osteogel, they provided additional osteoinductive cues in vitro that may pave the way for future
application in bone regeneration. MSCORS were also compared to MSCs from adipose tissue
(ADMSC) and bone marrow (BMMSC) in a 3D Osteogel model. We analyzed gel plasticity, cell
phenotype, cell viability, and differentiation capacity towards the osteogenic lineage by measuring
alkaline phosphatase (ALP) activity, calcium deposition, and specific gene expression. The novel
injectable hydrogel filled an irregularly shaped lesion in a porcine wound model displaying high
plasticity. MSCORS in Osteogel showed a higher osteo-commitment in terms of calcium deposition
and expression dynamics of OCN, BMP2, and PPARG when compared to ADMSC and BMMSC,
whilst displaying comparable cell viability and ALP activity. In conclusion, autologous MSCORS
combined with our novel gelatin-based hydrogel displayed a high capacity for differentiation towards
the osteogenic lineage and are acquired by non-invasive procedures, therefore qualifying as a suitable
and expandable novel approach in the field of bone regeneration therapy
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:88718 |
| Date | 19 December 2023 |
| Creators | Li, Hanluo, Nawaz, Hafiz Awais, Masieri, Federica Francesca, Vogel, Sarah, Hempel, Ute, Bartella, Alexander K., Zimmerer, Rüdiger, Simon, Jan-Christoph, Schulz-Siegmund, Michaela, Hacker, Michael, Lethaus, Bernd, Savković, Vuk |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 5404, 10.3390/ijms22105404 |
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