A microflow cytometer with simultaneous dielectrophoretic actuation for the optical assay and capacitive cytometry of individual fluid suspended bioparticles

Description

Fluid suspended biological particles (bioparticles) flowing through a non-uniform electric field are actuated by the induced dielectrophoretic (DEP) force, known to be dependent upon the bioparticles’ dielectric phenotypes. In this work: a 10-1000 kHz DEP actuation potential applied to a co-planar microelectrode array (MEA) induces a DEP force, altering passing bioparticle trajectories as monitored using: (1) an optical assay, in which the lateral bioparticle velocities are estimated from digital video; and (2) a capacitive cytometer, in which a 1.478 GHz capacitance sensor measures the MEA capacitance perturbations induced by passing bioparticles, which is sensitive to the bioparticles’ elevations. The experimentally observed and simulated lateral velocity profiles of actuated polystyrene microspheres (PSS) and viable and heat shocked Saccharomyces cerevisiae cells verify that the bioparticles’ dielectric phenotypes can be inferred from the resultant trajectories due to the balance between the DEP force and the viscous fluid drag force.

Links & Downloads

1. Ferrier, G.A., A.N. Hladio, D.J. Thomson, G.E. Bridges, M. Hedayatipoor, S. Olson, and M.R. Freeman (2007). Microfluidic electromanipulation with capacitive detection for cell diagnositc applications, Proc. NSTI 2:214-217.
2. Ferrier, G.A., S.F. Romanuik, D.J. Thomson, G.E. Bridges, and M.R. Freeman (2008). Capacitance signatures for rapid detection of the polarity of the dielectrophoretic force on single yeast cells, Proc. NSTI 2:589-592.
3. Ferrier, G.A., A.N. Hladio, D.J. Thomson, G.E. Bridges, M. Hedayatipoor, S. Olson, and M.R. Freeman (2008). Microfluidic electromanipulation with capacitive detection for the mechanical analysis of cells, Biomicrofluidics 2(4-044102):1-13.
4. Ferrier, G.A., S.F. Romanuik, D.J. Thomson, G.E. Bridges, and M.R. Freeman (Submitted 2009). A microwave interferometric system for simultaneous actuation and detection of single biological cells, Lab on a Chip.
5. Ferrier, G.A., S.F. Romanuik, D.J. Thomson, G.E. Bridges, and M.R. Freeman (Submitted 2009). Rapid classification of biological cells based on dielectrophoretic actuation with simultaneous capacitive detection, Proc. micro-TAS.
6. Nikolic-Jaric, M., S.F. Romanuik, G.A. Ferrier, G.E. Bridges, M. Butler, K. Sunley, D.J. Thomson, and M.R. Freeman (2009). Microwave frequency sensor for detection of biological cells in microfluidic channels, Biomicrofluidics 3(3-034103):1-15.
7. Romanuik, S.F., G.A. Ferrier, D.J. Thomson, G.E. Bridges, S. Olson, and M.R. Freeman (2008). All-electronic detection and actuation of single biological cells for lab-on-a-chip applications, Proc. IEEE Sensors 1:634-637.
8. Romanuik, S.F., G.A. Ferrier, M.N. Jaric, D.J. Thomson, G.E. Bridges, and M.R. Freeman (Submitted 2009). Dielectrophoretic actuation and simultaneous detection of individual bioparticles, Advances in Biomedical Sensing, Measurements, Instrumentation, and Systems, Eds. A. Lay-Ekuakille and S.C. Mukhopadhyay, Springer – Verlag.
9. http://hdl.handle.net/1993/3205

Tags

Microflow

Microfluidic

Cytometer

Cytometry

Dielectrophoretic

Dielectrophoresis

Interferometer

Interferometric

Capacitive Sensor

Capacitance Sensing

Capacitive Detector

Capacitance Detection

Polystyrene

Optical Assay

Electrokinetic

Actuation

Yeast

Saccharomyces cerevisiae

Single-cell Diagnostic

Single-cell Diagnosis

Dielectric Modeling

COMSOL

Tracker

Trajectory

Velocity Profile

Capacitive Signature

Capacitance Signature

Microelectrode

Additional Fields

Identifer	oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:MWU.1993/3205
Date	14 September 2009
Creators	Romanuik, Sean
Contributors	Thomson, Douglas (Electrical and Computer Engineering), Bridges, Gregory (Electrical and Computer Engineering) Lin, Francis (Physics and Astronomy)
Source Sets	Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
Language	en_US
Detected Language	English