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Previous issue date: 2007-08-15 / Massive resection of the small intestine results in short bowel syndrome with anti-absorptive effect and repercussions on the metabolism. Morphologic and functional evaluation may be necessary in order to control wrapped organs. Scintigraphy an examination with little invading and no biologic damage can be used. The purpose were to assess the biodistribution of sodium pertecnetate in organs of rats subjected to massive resection of the small intestine, the intestinal adaptation of the remnant intestinal mucosa and weight curve evaluation in the postoperative period. Twenty-one Wistar rats were randomly allocated into three groups (n = 7). The operated group named short bowel (SB) after anesthetized was subjected to massive resection of the small intestine; the control group (C), and sham group (SHAM). On the 30th postoperative day, 0.l mL of sodium pertechnetate was injected into the venous orbital plexus. After 30 minutes, the rats were killed with an overdose of anesthetic, and fragments of the liver, spleen, pancreas, stomach, duodenum, small intestine, thyroid, lung, heart, kidney, bladder, muscle, femur and brain were harvested. The percentage of radioactivity per gram of tissue (%ATI/g) was determined using Gama Counter WizardTM 1470, PerkinElmer to all samples. Biopsies of 3 cm remaining jejunum were removed to histological analyses. Analysis of variance (ANOVA) and the Tukey test for multiple comparisons were used, considering p<0.05 as significant. The study had the participation of some departments and laboratories, as Nucleus of Experimental Surgery, Department of Surgery, Laboratory of Radiobiology, Department of Pathology and Service of Nuclear Medicine, certifying the character of a multidisciplinary research. The results were no significant differences in %ATI/g of the sodium pertechnetate in the organs of the groups studied (p>0.05). An increase in the weight of the SB rats was observed after the second postoperative week. The jejunal mucosal thickness of the SB rats was significantly greater than that of C and sham rats (p<0.05. The biodistribution of sodium pertechnetate was not affected by massive intestinal resection in rats. An adaptive response by the intestinal mucosa probably contributed to the reversion of weight loss and the biodistribution of sodium pertechnetate was not affected by the surgery / A ressec??o extensa do intestino delgado resulta na s?ndrome do intestino curto com repercuss?o desabsortiva importante. Uma avalia??o morfofuncional poder? ser necess?ria aos ?rg?os envolvidos no processo. O objetivo desse estudo foi avaliar a biodistribui??o do pertecnetato de s?dio em ?rg?os de ratos submetidos a ressec??o extensa do intestino delgado, a capacidade adaptativa da mucosa intestinal remanescente e o comportamento da curva ponderal p?s-operat?ria. Foram utilizados 21 ratos Wistar alocados aleatoriamente em tr?s grupos (n=7). O grupo tratado, denominado intestino curto (IC), foi submetido a anestesia e ressec??o ampla do intestino delgado, o grupo controle (C) e o grupo sham que submeteu-se ? leve manipula??o cir?rgica das al?as intestinais. Ap?s observa??o por trinta dias administrou-se, em todos os grupos, 0,l mL de pertecnetato de s?dio via plexo venoso orbital. Decorridos trinta minutos os animais foram sacrificados com superdose de anest?sico para retirada de fragmentos do f?gado, ba?o, p?ncreas, est?mago, duodeno, intestino delgado, tire?ide, pulm?o, cora??o, rim, bexiga, m?sculo, f?mur, e c?rebro. As amostras foram levadas ao Contador Gama Autom?tico 1470, WizardTM, Perkin-Elmer para contagem da radioatividade e posterior c?lculo do percentual de atividade radioativa por grama (%ATI/g) em cada ?rg?o. Segmentos de 3cm do jejuno foram retirados para an?lise histol?gica da mucosa. Utilizou-se avalia??o estat?stica param?trica (ANOVA) e teste de Tukey, considerando p<0,05 como significante. N?o houve diferen?as significantes da %ATI/g nos ?rg?os dos grupos estudados. Verificou-se acentuada redu??o inicial de peso, em seguida um aumento do peso dos animais tratados a partir da segunda semana de observa??o e aumento da espessura da mucosa jejunal do grupo IC, comparado com os demais. Em conclus?o, a ressec??o ampla do intestino delgado n?o alterou a biodistribui??o do radiof?rmaco nos ?rg?os avaliados entre os animais estudados. A adapta??o da espessura da mucosa pode ter contribuido para revers?o na perda de peso p?s-operat?ria e para que a biodistribui??o do pertecnetato de s?dio n?o fosse afetada pela interven??o cir?rgica. O trabalho teve um car?ter multidisciplinar com a participa??o de v?rios departamentos e laborat?rios, como N?cleo de Cirurgia, Departamento de Cirurgia, Laborat?rio de Radiobiologia, Departamento de Patologia e Servi?o de Medicina Nuclear, atestando o car?ter multidisciplinar da pesquisa
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/13388 |
| Date | 15 August 2007 |
| Creators | Chacon, D?maso de Ara?jo |
| Contributors | CPF:07117701404, http://lattes.cnpq.br/9873289951810864, Fonseca, Adenilson de Souza da, CPF:02966851783, http://lattes.cnpq.br/8838215858149851, Catanho, Maria Teresa Jansem de Almeida, CPF:14967898491, http://lattes.cnpq.br/2204048396738679, Schwarzschild, L?cia de F?tima Campos Pedrosa, CPF:17547644449, http://lattes.cnpq.br/1863589790139155, Egito, Eryvaldo S?crates Tabosa do, CPF:38878240400, http://lattes.cnpq.br/6907806915889763, Medeiros, Aldo da Cunha |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Ci?ncias da Sa?de, UFRN, BR, Ci?ncias da Sa?de |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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