Cardioplegic arrest during heart operations is often used in cardiac surgery. During cardioplegia,
the heart is subjected to a global ischemia/reperfusion-injury. ()-epigallocatechin gallate (EGCG),
one of the main ingredients of green tea, seems to be beneficial in various cardiac diseases. Therefore,
the aim of our study was to evaluate EGCG in a rabbit model of cardioplegic arrest. Twenty four
mature Chinchilla rabbits were examined. Rabbit hearts were isolated and perfused according to
Langendorff. After induction of cardioplegia (without and with 20 mol/L EGCG, n = 6 each)
the hearts maintained arrested for 90-min. Thereafter, the hearts were re-perfused for 60 min.
During the entire experiment hemodynamic and functional data were assessed. At the end of each
experiment, left ventricular samples were processed for ATP measurements and for histological
analysis. Directly after cessation of cardioplegia, all hearts showed the same decline in systolic and
diastolic function. However, hearts of the EGCG-group showed a significantly faster and better
hemodynamic recovery during reperfusion. In addition, tissue ATP-levels were significantly higher
in the EGCG-treated hearts. Histological analysis revealed that markers of nitrosative and oxidative
stress were significantly lower in the EGCG group. Thus, addition of EGCG significantly protected
the cardiac muscle from ischemia/reperfusion injury.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:89414 |
| Date | 30 January 2024 |
| Creators | Salameh, Aida, Schuster, Roxana, Dähnert, Ingo, Seeger, Johannes, Dhein, Stefan |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 628 |
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