This thesis is concerned with biologically active substances in honey. The first part describes the characteristics of honey, its origin and its composition. It also deals with the polyphenols and importance of honey on human health. Polyphenols are the subject of many studies and was therefore chosen as representatives of the major biologically active substances. It describes their characteristics and representations of honey. The second part deals with the determination of polyphenols. For this purpose were selected 14 phenolic acids, which was determined in 8 kinds of honey. Analysis was performed by liquid chromatography mass spectrometry (HPLC-MS). Extraction was carried out by extraction by liquid-liquid and solid-phase extraction using two solvents, diethylether and ethylacetate. These methods were then statistically compared. The results show that the highest average content of phenolic acids was in honey pine honeydew and honeydew mixed. Generally, honeys contain most p-coumaric acid.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:250232 |
| Date | January 2015 |
| Creators | Strejčková, Aneta |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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