Water extracts of saffron, a spice derived from the plant Crocus Sativus L. obtained from India and Iran, were analyzed by liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS). The components in the extracts were separated on four different solid phase extraction cartridges with four different solvents and by silica gel column. Analysis was done by GC-MS, LC-MS and UV-Vis spectroscopy. The extracts separated into three distinct bands (two bright yellow and one orange) on the silica gel column. Based on GC-MS, the extracted compounds show many structural similarities. Using both extraction techniques, several compounds were identified that were not previously reported to be in saffron. Picrocrocin, safranal, and crocins presence in the extracts were evidenced by absorbance bands at wavelengths of 250 nm, 310 nm and 440 nm, respectively, in their UV-Vis spectra. The LC-MS analysis revealed several high molecular weight compounds that were not observed by GC-MS.
| Identifer | oai:union.ndltd.org:auctr.edu/oai:digitalcommons.auctr.edu:cauetds-1375 |
| Date | 31 July 2019 |
| Creators | Alsudairy, Ziad |
| Publisher | DigitalCommons@Robert W. Woodruff Library, Atlanta University Center |
| Source Sets | Atlanta University Center |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Electronic Theses & Dissertations Collection for Atlanta University & Clark Atlanta University |
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