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Previous issue date: 2017-06-23 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O c?ncer colorretal tem se destacado por ser um dos tumores mais freq?entes, com taxas de morbidade e mortalidade expressivos. Na descoberta de novas drogas, produtos derivados de plantas se destacam por ser uma fonte segura e capaz de originar compostos de alta efici?ncia. Bastante conhecida na medicina popular brasileira, Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea, tem sido utilizada no tratamento de um amplo espectro de condi??es e na preven??o do c?ncer. Nesse estudo, extratos etan?licos dos frutos de L. ferrea (a 20T, 40T, 60T e 80T) foram avaliados por 24 h e 48 h pela capacidade de inibi??o da prolifera??o celular; indu??o de apoptose atrav?s da avalia??o de Bcl-2, caspase-3 e Apaf-1; atividade antioxidante e efeito sobre alvos importantes relacionados a prolifera??o celular (EGFR e AKT) na linhagem colorretal humana HT-29, por meio de metodologias que envolveram ensaios de citometria de fluxo, espectrofotometria e RT-qPCR. Os resultados demostram que os extratos tiveram atividade antiproliferativa comparado ao controle, indu??o de apoptose atrav?s da via intr?nseca e a??o de inibi??o tumoral in vitro com a media??o de alvos importantes na tumorig?nese. Al?m disso, possui efeito antioxidante e anti-peroxida??o lip?dica, bem como quimioprotetor nas c?lulas saud?veis. Portanto, derivados de L. ferrea possuem importantes efeitos antic?ncer podendo ser considerados candidatos moleculares promissores para o tratamento do c?ncer colorretal. / Colorectal cancer is noted for being one of the most frequent of tumors, with expressive morbidity and mortality rates. In new drug discovery, plants stand out as a source capable of yielding safe and high-efficiency products. Well known in Brazilian popular medicine, Libidibia ferrea (Mart. Ex Tul.) L.P. Queiroz var. ferrea (better known as "ironwood" or "juc?"), has been used to treat a wide spectrum of conditions and to prevent cancer. Using methodologies that involved flow cytometry, spectrophotometry and RT-qPCR assays, ethanolic extracts of the fruits of L. ferrea (20T, 40T, 60T and 80T) were evaluated at 24 h and 48 h for: their ability to inhibit cell proliferation; induce apoptosis through Bcl-2, caspase-3 and Apaf-1; their antioxidant activity and effects on important targets related to cell proliferation (EGFR and AKT) in the HT-29 human colorectal cancer lineage. The results revealed antiproliferative activity as compared to the controls, induction of apoptosis through the intrinsic pathway, and in vitro tumor inhibition activity under the mediation of important targets in tumorigenesis. In addition, L. ferrea revealed antioxidant, lipid peroxidation and chemoprotective effects in healthy cells. Thus, L. ferrea derivatives have important anticancer effects, and may be considered promising candidate for colorectal cancer therapy.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/24000 |
| Date | 23 June 2017 |
| Creators | Guerra, Andreza Concei??o V?ras de Aguiar |
| Contributors | 01847881459, Vieira, Jeymesson Raphael Cardoso, 02930177438, Moura, S?rgio Adriane Bezerra de, 66398908449, Ara?jo J?nior, Raimundo Fernandes de |
| Publisher | PROGRAMA DE P?S-GRADUA??O EM CI?NCIAS DA SA?DE, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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