Optimized LC-MS/MS quantification method for the detection of piperacillin and application to the development of charged liposaccharides as penetration enhancers

Description

No / Piperacillin, a potent ß-lactam antibiotic, is effective in a large variety of Gram+ and Gram¿ bacterial infections but its administration is limited to the parenteral route as it is not absorbed when given orally. In an attempt to overcome this problem, we have synthesized a novel series of charged liposaccharide complexes of piperacillin comprising a sugar moiety derived from d-glucose conjugated to a lipoamino acid residue with varying side-chain length (cationic entity) and the piperacillin anion. A complete multiple reaction monitoring LC¿MS/MS method was developed to detect and characterize the synthesized complexes. The same method was then successfully applied to assess the in vitro apparent permeability values of the charged liposaccharide complexes in Caco-2 monolayers. / BBSRC

Links & Downloads

1. Violette, A., Cortes, D.F., Bergeon, J.A., Falconer, R.A. and Toth, I. (2008). Optimized LC-MS/MS quantification method for the detection of piperacillin and application to the development of charged liposaccharides as penetration enhancers. International Journal of Pharmaceutics.Vol. 351, Nos. 1-2, pp. 152-157.
2. http://hdl.handle.net/10454/4619
3. https://doi.org/10.1016/j.ijpharm.2007.09.039

Tags

Glycolipid

LC¿MS/MS

Piperacillin

Charged Liposaccharide

Penetration Enhancer

Permeability

Caco-2

Additional Fields

Identifer	oai:union.ndltd.org:BRADFORD/oai:bradscholars.brad.ac.uk:10454/4619
Date	January 2008
Creators	Violette, A., Cortes, D.F., Bergeon, J.A., Falconer, Robert A., Toth, I.
Source Sets	Bradford Scholars
Language	English
Detected Language	English
Type	Article, No full-text in the repository