Cell migration is an essential element in the immune response
on the one hand and in cancer metastasis on the other hand. The architecture
of the actin network in lamellipodia determines the elasticity of the leading
edge and contributes to the regulation of migration. We have implemented a
new method for the analysis of actin network morphology in the lamellipodia
of B16F1 mouse melanoma cells. This method is based on fitting multilayer
geometrical models to electron microscopy images of lamellipodial actin
networks. The chosen model and F-actin concentrations are thereby deterministic
parameters. Using this approach, we identified distinct structural features of
actin networks in lamellipodia. The mesh size which defines the elasticity of
the lamellipodium was determined as 34 and 78 nm for a two-layer network
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:80109 |
| Date | 25 July 2022 |
| Creators | Fleischer, Frank, Ananthakrishnan, Revathi, Eckel, Stefanie, Schmidt, Hendrik, Käs, Josef, Svitkina, Tatyana, Schmidt, Volker, Beil, Michael |
| Publisher | IOP Publishing |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 420 |
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