During the epithelial-to-mesenchymal transition, the intracellular cytoskeleton undergoes severe
reorganization which allows epithelial cells to transition into a motile mesenchymal phenotype.
Among the different cytoskeletal elements, the intermediate filaments keratin (in epithelial cells)
and vimentin (in mesenchymal cells) have been demonstrated to be useful and reliable histological
markers. In this study, we assess the potential invasiveness of six human breast carcinoma cell lines
and two mouse fibroblasts cells lines through single cell migration assays in confinement. We find
that the keratin and vimentin networks behave mechanically the same when cells crawl through
narrow channels and that vimentin protein expression does not strongly correlate to single cells
invasiveness. Instead, we find that what determines successful migration through confining spaces
is the ability of cells to mechanically switch from a substrate-dependent stress fibers based
contractility to a substrate-independent cortical contractility, which is not linked to their tumor
phenotype.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:85057 |
| Date | 02 May 2023 |
| Creators | Ficorella, Carlotta, Eichholz, Hannah Marie, Sala, Federico, Vázquez, Rebeca Martínez, Osellame, Roberto, Käs, Josef A. |
| Publisher | IOP Publishing |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 1367-2630, 083028 |
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