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Molekulárně-genetická analýza starokladrubských koní

The genetic variability and diversity of the Old Kladruber horse breed is evaluated in this master thesis. The aim is to evaluate the variability and diversity in the breed, between the sire lines and inside these lines. The detailed information about the Old Kladruber horse breed is presented in the literary overview with the breed history including the regeneration process. Basic indexes of the genetic variability are presented in the next part. 17 microsatellite markers were used to evaluate the genetic variability and diversity with further statistical analyses performed in GENEPOP 4.2 and ARLEQUIN 3.5 programs. The allele and genotype frequencies were calculated together with the number of alleles per loci and Hardy -- Weinberg equilibrium was tested for the population. Polymorphic information content, observed and expected heterozygosity and fixation coefficients were calculated. The results show surprisingly high degree of genetic variability in the Old Kladruber horse, increased probably due to introduction of other breeds during the regeneration process.

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:179075
Date January 2014
CreatorsVelebová, Adéla
Source SetsCzech ETDs
LanguageCzech
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/masterThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

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