Thesis (M.Sc.(Biochemistry)) -- University of Limpopo, 2020 / Metastatic cancer remains incurable and accounts for 90% of cancer-related deaths
(He et al., 2019). Therefore, there’s an urgent need to find anti-metastatic drugs with
novel therapeutic targets (Zhang et al., 2018). Medicinal plants are promising sources
of novel compounds with anti-metastatic activity (Tungsukruthai et al., 2018). This
study investigated the anti-metastatic effects of Momordica balsamina L. crude
acetone leaf extract in human HT-29 colon cancer cells. Powdered leaves of M.
balsamina were macerated in acetone and reconstituted in dimethyl sulfoxide
(>99.9%). The cytotoxic effect of the M. balsamina extract was investigated using the
MTT assay. The acridine orange/ethidium bromide dual staining assay was used to
show that the chosen concentrations of the M. balsamina extract do not induce
apoptosis. The effect of the M. balsamina extract on reactive oxygen species formation
and epithelial to mesenchymal transition-related morphological changes were
assessed by the DCFH2-DA assay and light microscopy, respectively. The
anti-invasive, anti-migratory and anti-adhesive effects of the M. balsamina extract
were investigated using the cell invasion, wound-healing and cell adhesion assays,
respectively. The adhesion of HT-29 cells to collagen I, II and IV, fibronectin, laminin,
tenascin C and vitronectin was assessed using the ECM-cell adhesion array kit.
Furthermore, western blotting was used to assess the effect of the M. balsamina
extract on the expression of TNF-α, NF-κB, MMP-2, MMP-9 and TIMP-3. The findings
revealed that the M. balsamina extract significantly inhibited the viability of HT-29 cells
at concentrations above 50 µg/ml but had no effect on the viability of C3A liver cells at
40 and 80 µg/ml. Apoptotic features such as cell shrinkage, nuclei condensation, loss
of membrane function and formation of apoptotic bodies were observed at 48 hours
exposure to the M. balsamina extract. Reactive oxygen species formation, epithelial
to mesenchymal transition, invasiveness, migration and adhesion were suppressed in
HT-29 cells treated with the M. balsamina extract for 24 hours. The adhesion of
HT-29 cells were varied amongst different extracellular matrix proteins. Furthermore,
HT-29 cells treated with the M. balsamina extract showed a reduction in the expression
of TNF-α, NF-κB, MMP-2 and MMP-9 proteins and an upregulation of TIMP-3 protein.
In conclusion, the M. balsamina extract tested in this study impedes the metastatic
cascade in HT-29 colon cancer cells by inhibiting the reactive oxygen
species-mediated TNF-α/NF-κB/MMP-2/MMP-9 pathway. The findings suggest that M. balsamina L. may be a source of compounds with potential therapeutic use for the
treatment of metastatic colon cancer. / National Research Foundation (NRF)
South African Medical Research Council (SAMRC)
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:ul/oai:ulspace.ul.ac.za:10386/3509 |
| Date | January 2020 |
| Creators | Serala, Karabo |
| Contributors | Mbazima, V. G., Mampuru, L. J., Prince, S. |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | ix, 68 leaves |
| Relation |
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