Current screening tests for type 2 diabetes mellitus (T2DM) identify less than 50% of
undiagnosed T2DM patients and provide no information about how the disease will develop in
prediabetic patients. Here, twenty-nine protein glycation sites were quantified after tryptic digestion of
plasma samples at the peptide level using tandem mass spectrometry and isotope-labelled peptides
as internal standard. The glycation degrees were determined in three groups, i.e., 48 patients with a
duration of T2DM exceeding ten years, 48 non-diabetic individuals matched for gender, BMI, and age,
and 20 prediabetic men. In long-term controlled diabetic patients, 27 glycated peptides were detected at
significantly higher levels, providing moderate diagnostic accuracies (ACCs) from 61 to 79%, allowing
a subgrouping of patients in three distinct clusters. Moreover, a feature set of one glycated peptides
and six established clinical parameters provided an ACC of 95%. The same number of clusters was
identified in prediabetic males (ACC of 95%) using a set of eight glycation sites (mostly from serum
albumin). All patients present in one cluster showed progression of prediabetic state or advanced
towards diabetes in the following five years. Overall, the studied glycation sites appear to be promising
biomarkers for subgrouping prediabetic patients to estimate their risk for the development of T2DM.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:84602 |
| Date | 06 April 2023 |
| Creators | Spiller, Sandro, Li, Yichao, Blüher, Matthias, Welch, Lonnie, Hoffmann, Ralf |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 1424-8247, 38 |
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