<p dir="ltr">Mass spectrometry imaging (MSI) is a powerful technique for visualizing the distribution of molecules within biological samples. Advancements in MSI instrumentation and computational tools have enabled the impactful applications of this technique across various fields including clinical research, drug discovery, forensics, microbiology, and natural products. Nanospray desorption electrospray ionization (nano-DESI), an ambient localized liquid extraction ionization technique, has proven valuable to the MSI community. Nano-DESI has been used for imaging of various molecules in biological samples including drugs, metabolites, lipids, N-linked glycans, and proteins.</p><p dir="ltr">My research has been focused on expanding the sensitivity and specificity of nano-DESI for biomolecular imaging. One of the newly developed methods employs ammonium fluoride NH<sub>4</sub>F as a solvent additive to enhance the sensitivity of nano-DESI for the analysis of lipids in negative ionization mode. Secondly, methods were developed for the spatial mapping of isobaric and isomeric species in biological tissues by implementing nano-DESI MSI on a triple quadrupole (QqQ) mass spectrometer. This work used multiple reaction monitoring (MRM) mode of a QqQ with unit mass resolution to separate isobaric lipid species that require high mass resolving power and imaging of isomeric low-abundance species in tissue sections. Next, I demonstrate nano-DESI as a liquid extraction technique for imaging of N-linked glycans within biological tissue sections. Lastly, the spatial distribution of eicosanoids and specialized pro-resolving mediators (SPMs) in a mouse model for acetaminophen-induced liver injury (AILI) provides insights into the inflammation and resolution phases of AILI. Collectively, these developments have advanced the sensitivity, chemical specificity, and molecular coverage of nano-DESI for imaging of different classes of molecules in biological tissues.</p>
| Identifer | oai:union.ndltd.org:purdue.edu/oai:figshare.com:article/26337040 |
| Date | 19 July 2024 |
| Creators | Miranda Renee Weigand (19179571) |
| Source Sets | Purdue University |
| Detected Language | English |
| Type | Text, Thesis |
| Rights | CC BY 4.0 |
| Relation | https://figshare.com/articles/thesis/_b_ADVANCEMENTS_IN_AMBIENT_MASS_SPECTROMETRY_IMAGING_FOR_ENHANCED_SENSITIVITY_AND_SPECIFICITY_OF_COMPLEX_BIOLOGICAL_TISSUES_b_/26337040 |
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