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Previous issue date: 2011-02-16 / Periodontal disease is an infection initiated by oral periodontal pathogens that trigger
an immune response culminating in tissue destruction. This destruction is mediated by the
host by inducing the production and activation of lytic enzymes, cytokines and the stimulation
of osteoclastogenesis. The aim of this study was to compare the immunohistochemical
expression of factors involved in bone resorption, RANKL (Ligand Receptor Activator of
Nuclear Factor kappa B), OPG (Osteoprotegerin) and TNF-α (tumor necrosis factor alpha)
between the gingival healthy, gingivitis and chronic periodontitis and correlate them with
clinical parameters. The sample consisted of 83 cases and 12 clinically healthy gums, 42
gingivitis and 29 periodontitis, from 74 adolescent and adult patients with a mean age of 35
years, without systemic changes and non-smokers, predominantly female and race brown.
There was no statistically significant difference for the expression of anti-RANKL (p = 0.581)
and RANKL / OPG ratio (p = 0.334) when comparing the three conditions, but the anti-OPG
and anti-TNF-α showed statistically significant between the types of injury (p = 0.001 and p
<0.001, respectively), showing greatest expression in periodontitis. In cases of periodontitis,
the variable clinical attachment loss (PIC) was statistically significant and positive
correlation, respectively, with immunostaining of anti-RANKL (p = 0.002, p = 0.001 and r =
0.642), anti-OPG (p = 0.018, p = 0.014 and r = 0.451), anti-TNF-α (p = 0.032, p = 0.014 and r
= 0.453) and the percentage ratio of RANKL / OPG (p = 0.018, p = 0.002 and r = 0.544). The
tooth mobility (MB) showed a statistically significant difference only with
immunohistochemical anti-RANKL (p = 0.026), and probing depth (PD) was positively
correlated with anti-RANKL (p = 0.028 and r = 0.409), both in cases of periodontitis. Only in
cases of gingivitis TNF-α was positively correlated with RANKL (p = 0.012 and r = 0.384)
and the RANKL / OPG ratio (p = 0.027 and r = 0.341). Given these results, we conclude that
the greatest expression of TNF-α in periodontitis demonstrates a relationship with the
progression and severity of periodontal disease and the correlation between all antibodies and
clinical attachment loss demonstrates their involvement in periodontal bone resorption / A doen?a periodontal ? uma infec??o oral iniciada por periodontopat?genos que
desencadeiam a resposta imune culminando com a destrui??o tecidual. Essa destrui??o ?
mediada pelo hospedeiro atrav?s da indu??o da produ??o e ativa??o de enzimas l?ticas,
citocinas e da estimula??o da osteoclastog?nese. O objetivo deste estudo foi comparar a
express?o imuno-histoqu?mica dos fatores envolvidos na reabsor??o ?ssea, RANKL (Ligante
do Receptor Ativador do Fator Nuclear kappa B), OPG (Osteoprotegerina) e TNF-α (Fator de
Necrose Tumoral Alfa) entre a gengiva clinicamente saud?vel, a gengivite e a periodontite
cr?nica, correlacionando-os com os par?metros cl?nicos periodontais. A amostra consistiu de
83 casos, sendo 12 de gengivas clinicamente saud?veis, 42 de gengivite e 29 de periodontite,
oriundos de 74 pacientes adolescentes e adultos com idade m?dia de 35 anos, sem altera??es
sist?micas e n?o fumantes, predominantemente do sexo feminino e da ra?a parda. N?o houve
diferen?a estatisticamente significativa para express?o do anticorpo anti-RANKL (p=0,581) e
da raz?o RANKL/OPG (p=0,334) quando se comparou as tr?s condi??es cl?nicas, mas o anti-
OPG e anti-TNF-α mostraram diferen?as estatisticamente significativas entre os tipos de les?o
(p=0,001 e p<0,001, respectivamente), revelando maior imunoexpress?o na periodontite. Nos
casos de periodontite, a vari?vel perda de inser??o cl?nica (PIC) mostrou diferen?a
estatisticamente significativa e correla??o positiva, respectivamente, com a imunomarca??o
dos anticorpos anti-RANKL (p=0,002; p=0,001 e r=0,642), anti-OPG (p=0,018; p=0,014 e
r=0,451), anti-TNF-α (p=0,032; p=0,014 e r=0,453) e com a raz?o percentual de
RANKL/OPG (p=0,018; p=0,002 e r=0,544). A mobilidade dent?ria (MB) apresentou
diferen?a estatisticamente significativa somente com a imunoexpress?o do anti-RANKL
(p=0,026), e a profundidade de sondagem (PS) apresentou correla??o positiva com o anti-
RANKL (p=0,028 e r=0,409), ambos nos casos de periodontite. Somente nos casos de
gengivite o TNF-α apresentou correla??o positiva com o RANKL (p=0,012 e r=0,384) e com
a raz?o RANKL/OPG (p=0,027 e r=0,341). Diante desses resultados, conclui-se que a maior
imunoexpress?o do TNF-α na periodontite demonstra uma rela??o com a progress?o e
severidade da doen?a periodontal e a correla??o entre todos os anticorpos e a perda de
inser??o cl?nica demonstra o envolvimento destes na reabsor??o ?ssea periodontal
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/17150 |
| Date | 16 February 2011 |
| Creators | Amaral, Bruna Aguiar do |
| Contributors | CPF:32381611487, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786083Z7&dataRevisao=null, Lins, Ruthn?ia Di?genes Alves Uch?a, CPF:87808838420, http://lattes.cnpq.br/4462076152744400, Pinto, Le?o Pereira, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787928A2, Miguel, M?rcia Cristina da Costa, CPF:82134731400, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707501Z3&dataRevisao=null, Costa, Ant?nio de Lisboa Lopes |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Patologia Oral, UFRN, BR, Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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