Normothermic machine perfusion (NMP) allows for ex vivo viability and functional
assessment prior to liver transplantation (LT). Hyperspectral imaging represents a
suitable, non-invasive method to evaluate tissue morphology and organ perfusion
during NMP. Liver allografts were subjected to NMP prior to LT. Serial image
acquisition of oxygen saturation levels (StO2), organ hemoglobin (THI), near-infrared
perfusion (NIR) and tissue water indices (TWI) through hyperspectral imaging was
performed during static cold storage, at 1h, 6h, 12h and at the end of NMP. The
readouts were correlated with perfusate parameters at equivalent time points. Twentyone
deceased donor livers were included in the study. Seven (33.0%) were discarded due
to poor organ function during NMP. StO2 (p < 0.001), THI (p < 0.001) and NIR (p = 0.002)
significantly augmented, from static cold storage (pre-NMP) to NMP end, while TWI
dropped (p = 0.005) during the observational period. At 12–24h, a significantly higher
hemoglobin concentration (THI) in the superficial tissue layers was seen in discarded,
compared to transplanted livers (p = 0.036). Lactate values at 12h NMP correlated
negatively with NIR perfusion index between 12 and 24h NMP and with the delta NIR
perfusion index between 1 and 24h (rs = −0.883, p = 0.008 for both). Furthermore, NIR and
TWI correlated with lactate clearance and pH. This study provides first evidence of feasibility of hyperspectral imaging as a potentially helpful contact-free organ viability
assessment tool during liver NMP.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:86357 |
| Date | 03 July 2023 |
| Creators | Fodor, Margot, Lanser, Lukas, Hofmann, Julia, Otarashvili, Giorgi, Pühringer, Marlene, Cardini, Benno, Oberhuber, Rubert, Resch, Thomas, Weissenbacher, Annemarie, Maglione, Manuel, Margreiter, Christian, Zelger, Philipp, Pallua, Johannes D., Öfner, Dietmar, Sucher, Robert, Hautz, Theresa, Schneeberger, Stefan |
| Publisher | Frontiers Media |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 1432-2277, 10355 |
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