Thesis (MSc (Biochemistry))--Stellenbosch University, 2008. / This study describes:
1. The preparation of chloroform, methanol and aqueous extracts of
unfermented and fermented rooibos (Aspalathus linearis).
2. The chromatographic fractionation of aqueous rooibos extracts and an
investigation into the polyphenol content and antioxidant activity of
the fractions.
3. The preparation of ovine adrenal microsomes containing active
steroidogenic P450 enzymes, including cytochrome P450
17a-hydroxylase, CYP17, and cytochrome P450 steroid
21-hydroxylase, CYP21.
4. An investigation into the influence of chloroform and methanol
extracts of rooibos on the binding of steroid substrates, progesterone
and 17-hydroxyprogesterone, to CYP17 and CYP21.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:sun/oai:scholar.sun.ac.za:10019.1/3020 |
| Date | 03 1900 |
| Creators | Richfield, David |
| Contributors | Swart, A. C., Swart, P., Stellenbosch University. Faculty of Science. Dept. of Biochemistry. |
| Publisher | Stellenbosch : Stellenbosch University |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Rights | Stellenbosch University |
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