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Previous issue date: 2011-08-30 / Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq / Paracoccidioides brasiliensis is a dimorphic fungus, the causative agent of paracoccidioidomycosis (PCM), a systemic mycosis that affects mainly people from Latin America. In order to establish infection, many factors are required for P. brasiliensis, such as cell wall remodeling and the ability to capture nutrients from the host. As iron is an important cofactor of many reactions, the host reduces the availability of free iron ions to the pathogen. Therefore, this study aims to elucidate the proteomic profile of the cell wall in response to iron depletion. The extraction of surface proteins of the cell wall in iron restriction and iron replete conditions was performed and the proteomic profile was obtained by two-dimensional electrophoresis with subsequent analysis of images. Statistical analysis demonstrated proteins with differential level of expression in each condition, which were subjected to tryptic digestion and identification by mass spectrometry. It was identified 26 proteins by peptide mass fingerprint (PMF) and/or ions fragmentation (MS/MS). The majority of these proteins have post - translational modifications and only some have in their sequences signal peptide, suggesting that they are addressed to the surface by non-classical pathways. / Paracoccidioides brasiliensis é um fungo patogênico termodimórfico, agente causador da Paracoccidioidomicose (PCM), uma micose sistêmica que afeta principalmente indivíduos da América Latina. Muitos fatores são necessários para que P. brasiliensis estabeleça infecção, como o remodelamento da parede celular e a capacidade de captar nutrientes do hospedeiro para seu metabolismo. Como o íon ferro é um cofator de diversas reações, o hospedeiro diminui a disponibilidade de íons ferro na forma livre para o patógeno. Portanto, o presente trabalho visa investigar o perfil de proteínas da parede celular de P. brasiliensis quando em condição de depleção do íon ferro. Para esse fim, realizou-se a extração das proteínas da parede das células de P. brasiliensis em meio com restrição do íon ferro. O perfil proteômico das condições experimental e controle foram obtidos por eletroforese bidimensional com posterior análise de imagens. Análises estatísticas demonstraram as proteínas reguladas nas condições de depleção de ferro, que foram submetidas à digestão tríptica e identificação por espectrometria de massas. Foram identificadas 26 proteínas por perfil de digestão tríptica (PMF) e/ou perfil de fragmentação dos íons (MS/MS). A maioria dessas proteínas apresenta preditas modificações pós - traducionais e apenas algumas apresentam peptídeo sinal em suas sequências, o que sugere que elas são endereçadas à superfície por vias não clássicas.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.bc.ufg.br:tede/3222 |
| Date | 30 August 2011 |
| Creators | Philippsen, Hellen Kempfer |
| Contributors | Soares, Célia Maria de Almeida, Soares, Célia Maria de Almeida, Kipnis, André, Pereira, Maristela, Bailão, Alexandre Melo, Borges, Clayton Luís |
| Publisher | Universidade Federal de Goiás, Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP), UFG, Brasil, Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG) |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | Portuguese |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFG, instname:Universidade Federal de Goiás, instacron:UFG |
| Rights | http://creativecommons.org/licenses/by-nc-nd/4.0/, info:eu-repo/semantics/openAccess |
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