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Previous issue date: 2015-07-20 / O pericarpo de Passiflora edulis var. flavicarpa Degener atualmente est? sendo investigado
para fins medicinais. N?o h? relatos na literatura sobre sua toxicidade. O objetivo do presente
estudo foi investigar a toxicidade sub-cr?nica em ratos machos e toxicidade em ratas f?meas
prenhes e fetos expostos durante a gesta??o a um extrato obtido por infus?o do pericarpo em
?gua (1:3 m/v; 100o C, 10 min). A composi??o do extrato foi avaliada por meio de rea??es
qu?micas e tamb?m por cromatografia de camada delgada. Ratos machos adultos (n=8) foram
tratados com 300 mg/kg de extrato, por gavagem, durante 30 dias e ratas prenhes (n=7) do dia
0 ao dia 20 de gesta??o. Os grupos controle de ratos machos (n=8) e ratas f?meas (n=7)
receberam ?gua pot?vel (1 ml). Durante o tratamento foram registrados o consumo de ?gua e
de ra??o e o peso corporal. No dia 29 o comportamento sexual dos machos foi analisado e, em
seguida, metade dos machos de cada grupo receberam ciclofosfamida (50 mg/kg, i.p.) para
avalia??o (anti) genot?xica na medula ?ssea e a outra metade recebeu apenas solu??o salina
0,9% (1 mL, i.p.). No dia 30, os machos foram anestesiados para coleta de sangue, medula
?ssea e ?rg?os. No dia 20 de gesta??o, as ratas prenhes foram anestesiadas para avalia??o do
desempenho reprodutivo. A an?lise dos fetos foi realizada por exame visceral e esquel?tico. A
an?lise fitoqu?mica revelou a presen?a de flavon?ides, alcal?ides inespec?ficos, compostos
fen?licos e triterp?nicos. A an?lise estat?stica revelou aus?ncia de diferen?as em todos os
par?metros analisados para machos, para f?meas e para fetos experimentais em rela??o aos
animais controle. A citotoxicidade foi avaliada pela rela??o entre eritr?citos policrom?ticos e
normocrom?ticos (EPC/ENC). A an?lise estat?stica (m?dia ? erro do desvio padr?o) revelou
aus?ncia de altera??es na frequ?ncia de EPCMN (controle negativo: 3,26?0,42; controle
positivo: 11,72?1,02; experimental negativo: 4,02?0,13; experimental positivo: 10,47?0,87)
ou citotoxicidade (controle negativo: 0,37?0,08; controle positivo: 0,23?0,05; experimental
negativo: 0,37?0,07; experimental positivo: 0,23?0,02). Este estudo sugere que o extrato
aquoso obtido de pericarpo de P. edulis var. flavicarpa Degener n?o foi capaz de promover
efeitos t?xicos em ratos nas condi??es utilizadas. / The pericarp of Passiflora edulis var. flavicarpa Degener is now being investigated for
medicine purposes. There are no reports about it toxicity. The aim of the present study was
investigate the sub chronic toxicity in male rats and reproductive toxicity in pregnant rats and
exposed fetuses of an extract obtained by infusion of the pericarp in water (1:3 m/v;100o C, 10
min). The extract composition was evaluated by tube reactions and thin lawyer
chromatography (TLC). Adult male rats (n=8) were treated with 300 mg/kg of the extract, by
gavage, during 30 days and pregnant rats (n=7) from gestation day 0 to day 20. Control
received tap water (1 mL). Water and food intakes and body weight gain were recorded. At
day 29 of treatment the sexual behavior of the males was analyzed and then half of males
from each group received cyclophosphamide (50 mg/kg, i.p.) to (anti)genotoxic assessment in
bone marrow. At day 30, males were anesthesized for parameters collection. At day 20 of
gestation, the dams were anesthesized for reproductive performance evaluation. The fetal
analysis was conducted by visceral and skeletal. Phytochemical analysis revealed the presence
of flavonoids, unspecific alkaloids, phenols and triterpenic compounds. Statistical analysis
revealed absence of significant differences between experimental and control. This study
suggest that the aqueous extract obtained from pericarp of P. edulis var. flavicarpa Degener
was not able to promote toxic effects in rats. Cytotoxicity was evaluated with the PCE/NCE
ratio (NCE=normochromatic erythrocytes). Statistical analysis (mean ? SEM) revealed
absence of changes in the frequency of MNPCE (negative control: 3.26?0.42; positive
control: 11.72?1.02; negative experimental: 4.02?0.13; positive experimental: 10.47?0.87) or
cytotoxicity (negative control: 0.37?0.08; positive control: 0.23?0.05; negative experimental:
0.37?0.07; positive experimental: 0.23?0.02). This study suggests that the extracts showed
no (anti)genotoxic and no cytotoxic activities under the experimental conditions.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/20302 |
| Date | 20 July 2015 |
| Creators | Arruda, Andr?a Caroline Costa de |
| Contributors | 03065039648, http://lattes.cnpq.br/2401319567715548, Luchessi, Andr? Ducati, 30489740839, http://lattes.cnpq.br/4420863418928278, Carvalho, Virginia Martins, 25242561890, http://lattes.cnpq.br/3629850103370810, Langassner, Silvana Maria Zucolotto, Schwarz, Aline |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM CI?NCIAS FARMACEUTICAS, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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