The subthalamic nucleus (STN) is a key structure for motor, limbic and associative function. Parkinson's disease (PD) and Obsessive compulsive disorder (OCD) have been connected to abnormal firing activity of STN neurons. Subthalamotomy and deep brain stimulation (DBS) of the STN have been shown to be effective treatment methods for previous suggested diagnosis, however, the mechanism behind the treatment and the reason for adverse side-effects remains unsolved. The aim of this study was to establish if it is possible to distinguish specific gene expressions in structures of transgenic mouse brains as well as gaining more knowledge about the medial part of STN, which is hypothesized to be the limbic part of the structure. Here, immunofluorescence and PCR was performed on Cre-driven transgenic mice followed by histological analysis in order to identify the distribution of tachykinin 1 (Tac1) and paired-like homeodomain 2 (Pitx2) in these transgenic mice brains. The results demonstrate that STN and pSTN were positive structures in Pitx2-CreSunGFP and Tac1-CreSunGFP mice. Tac1-CreSunGFP mice further contained GFP positive cells in striatum, nucleus accumbens, amygdala, thalamus, hypothalamus, submedial nucleus of thalamus and the septal area, proposing the gene expression of Tac1 to be present in several limbic structures. Today, we lack knowledge of the internal organization of the STN, and solving the structural-functional organization of the STN would be helpful to distinguish distinct roles of STN neurons and develop effective STN-DBS-targeting treatment without side-effects.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:uu-503722 |
| Date | January 2023 |
| Creators | Poska Lund, Noomi |
| Publisher | Uppsala universitet, Institutionen för biologisk grundutbildning |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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