by Law Shui Chee Annie. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1997. / Includes bibliographical references (leaves 117-125). / Abstract --- p.i / Abbreviations --- p.iii / List of Figures --- p.iv / List of Tables --- p.vi / Chapter 1. --- INTRODUCTION --- p.1 / Chapter 1.1 --- Background of Pleurotus pulmonarius --- p.1 / Chapter 1.1.1 --- Taxonomy and classification --- p.1 / Chapter 1.1.2 --- Life cycle --- p.2 / Chapter 1.1.3 --- Marketing value --- p.4 / Chapter 1.1.4 --- Nutritional content --- p.4 / Chapter 1.2 --- Background of calcium --- p.5 / Chapter 1.2.1 --- Calcium requirements for human --- p.6 / Chapter 1.2.2 --- The biological role of calcium --- p.6 / Chapter 1.2.3 --- Calcium as a regulator --- p.8 / Chapter 1.2.4 --- Binding of calcium --- p.9 / Chapter 1.2.5 --- Uptake of calcium --- p.11 / Chapter 1.2.6 --- Calcium transport --- p.11 / Chapter 1.2.7 --- Application of calcium on mushroom cultivation --- p.12 / Chapter 1.2.8 --- Calcium effect on mushroom --- p.13 / Chapter 1.3 --- Background of manganese --- p.13 / Chapter 1.3.1 --- The biological role of manganese --- p.14 / Chapter 1.3.2 --- Uptake of manganese --- p.15 / Chapter 1.3.3 --- Manganese requirements of humans --- p.16 / Chapter 1.3.4 --- Manganese deficiency --- p.16 / Chapter 1.3.5 --- Use of manganese --- p.17 / Chapter 1.3.6 --- Influence of pH on manganese toxicity --- p.17 / Chapter 1.3.7 --- Effects of manganese on enzymes --- p.18 / Chapter 1.3.8 --- Application of manganese on mushroom cultivation --- p.19 / Chapter 1.4 --- Purpose of Study --- p.19 / Chapter 2. --- MATERIALS AND METHODS --- p.21 / Chapter 2.1 --- Organisms --- p.21 / Chapter 2.2 --- Maintenance of cultures --- p.21 / Chapter 2.3 --- Identification of two strains --- p.21 / Chapter 2.3.1 --- Determination of growth rate --- p.22 / Chapter 2.3.2 --- Arbitrarily-primed polymerase chain reaction (AP-PCR) --- p.22 / Chapter 2.3.3 --- Mating type reaction --- p.25 / Chapter 2.4 --- Effect of different concentrations of calcium and manganese on the life cycle of the fungi --- p.26 / Chapter 2.4.1 --- Spore germination --- p.26 / Chapter 2.4.2 --- Preparation of mycelium homogenate --- p.27 / Chapter 2.4.3 --- Vegetative growth --- p.28 / Chapter 2.4.4 --- Fruiting initiation --- p.30 / Chapter 2.4.5 --- Fruiting --- p.31 / Chapter 2.4.6 --- Fruiting yield (Biological efficiency) --- p.32 / Chapter 3. --- RESULTS --- p.35 / Chapter 3.1 --- Identification of two strains --- p.35 / Chapter 3.1.1 --- Determination of growth rate --- p.35 / Chapter 3.1.2 --- Fruitbody morphology --- p.35 / Chapter 3.1.3 --- Arbitrarily primed polymerase chain reaction (AP-PCR) --- p.35 / Chapter 3.1.4 --- Mating type reaction --- p.40 / Chapter 3.2 --- Effect of calcium and manganese on the life cycle of the fungus --- p.40 / Chapter 3.2.1 --- Spore germination --- p.40 / Chapter 3.2.2 --- Vegetative growth --- p.44 / Chapter 3.2.3 --- Fruiting initiation in vitro --- p.58 / Chapter 3.2.4 --- Fruiting --- p.58 / Chapter 4. --- DISCUSSION --- p.96 / Chapter 4.1 --- Effects of calcium and manganese on spore germination --- p.96 / Chapter 4.2 --- Effects of calcium and manganese on vegetative growth --- p.97 / Chapter 4.2.1 --- Biomass study --- p.97 / Chapter 4.2.2 --- Glucose utilization --- p.99 / Chapter 4.2.3 --- Protein secretion --- p.99 / Chapter 4.2.4 --- Orthophosphate utilization --- p.102 / Chapter 4.2.5 --- Ammonia content study --- p.104 / Chapter 4.2.6 --- Metal content study --- p.106 / Chapter 4.3 --- Effects of calcium and manganese on fruiting initiation --- p.107 / Chapter 4.4 --- Effects of calcium and manganese on fruiting --- p.107 / Chapter 4.4.1 --- Fruiting yield (biological efficiency) --- p.109 / Chapter 4.4.2 --- Metal content in fruitbodies --- p.109 / Chapter 4.4.3 --- "Carbon, hydrogen, nitrogen and sulfur contents in fruitbodies" --- p.111 / Chapter 4.4.3 --- Amino acid content in fruitbodies --- p.114 / Chapter 4.5 --- Response of different stages towards metals --- p.115 / Chapter 5. --- CONCLUSION --- p.116 / Chapter 6. --- REFERENCES --- p.117 / Chapter 7. --- APPENDIX --- p.126 / Chapter 7.1 --- Preparation of reagents for determination of orthophosphate content --- p.126 / Chapter 7.1.1 --- Stock standard 100.0 mg P/L --- p.126 / Chapter 7.1.2 --- Working stock standard solution 10.0 mg P/L --- p.126 / Chapter 7.1.3 --- Stock ammonium molybdate solution --- p.126 / Chapter 7.1.4 --- Stock antimony potassium tartrate solution --- p.126 / Chapter 7.1.5 --- Molybdate color reagent --- p.126 / Chapter 7.1.6 --- Ascorbic acid reducing solution --- p.127 / Chapter 7.1.7 --- Sodium hydroxide-EDTA rinse --- p.127 / Chapter 7.2 --- Preparation of reagents for determination of ammonia content --- p.127 / Chapter 7.2.1 --- Stock standard 100.0 mgN/L as NH3 in 2 M KC1 --- p.127 / Chapter 7.2.2 --- Working standards --- p.127 / Chapter 7.2.3 --- Potassium chloride and standards diluent --- p.127 / Chapter 7.2.4 --- EDTA solution --- p.127 / Chapter 7.2.5 --- Buffer --- p.127 / Chapter 7.2.6 --- Salicylate-nitroprusside color reagent recipe --- p.128 / Chapter 7.2.7 --- Hypochlorite reagent --- p.128
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_321866 |
| Date | January 1997 |
| Contributors | Law, Shui Chee Annie., Chinese University of Hong Kong Graduate School. Division of Biology. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, vii, 128 leaves : ill. (some mounted) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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