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Physiological changes and responses of pseudomonas aeruginosa ATCC 9027 when grown on petroleum compounds

Physiological and compositional changes in Pseudomonas aeruginosa (ATCC 9027) were monitored during, growth on various petroleum compounds in a chemically-defined medium. Growth of P. aeruginosa was observed when furnace oil, kerosene, aviation fuel, light crude oil and hexadecane were used as carbon and energy sources. A variable and extended lag period before active growth was achieved was characteristic of petroleum-grown cells as compared to glucose-grown cells. Growth on the petroleum hydrocarbons, compared with that on glucose, resulted in changes in cell lipid composition, outer membrane proteins, cell-surface hydrophobicity, surface-tension, and pH changes in the growth medium during transition from early to late-log phase. Cell composition and physiology of cells grown in the petroleum mixtures varied due to differences in the chemical composition of the material. Production of an exopolymer (characterized as a peptidoglycolipid) was associated with petroleum-grown cells but not with glucose-grown cells. The above differences illustrate some of the dynamic and physiological and biochemical changes the microorganism undergoes to access its hydrophobic carbon and energy source.

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.42117
Date January 1997
CreatorsPietrantonio, Frank A.
ContributorsGreer, C. W. (advisor)
PublisherMcGill University
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
LanguageEnglish
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Formatapplication/pdf
CoverageDoctor of Philosophy (Department of Natural Resource Sciences.)
RightsAll items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated.
Relationalephsysno: 001567335, proquestno: NQ30359, Theses scanned by UMI/ProQuest.

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