The capability to control the progression of the cell cycle, including the means to enter into a stable non-proliferative state, is essential for eukaryotic unicellular and multicellular organisms. A quiescent state similar to G0 of higher eukaryotes can be induced by nitrogen starvation of the fission yeast model organism Schizosaccharomyces pombe. Using high-resolution tiling arrays for genome-wide transcriptional profiling we explore the early transcriptional reprogramming on the route to quiescence. Furthermore, we demonstrate that cells carrying a mutation in the high mobility group (HMG) box of the histone lysine demethylase spLsd1 fail to acquire characteristics of quiescent cells and rapidly lose viability under nitrogen-starved conditions. Since no such defect is seen as a result of catalytic inactivation, the HMG domain of spLsd1 seems to confer a function to the protein that is independent of the histone demethylase activity. We show that the HMG domain of spLsd1 is required for transcriptional activation and repression of a large set of genes, both during vegetative growth and on the route to quiescence. We also confirm that spLsd1 is a repressor of antisense transcription, and that this function is at least partially dependent on the HMG domain of the protein.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:sh-1772 |
| Date | January 2008 |
| Creators | Norman, Ulrika |
| Publisher | Södertörns högskola, Institutionen för livsvetenskaper |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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