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Previous issue date: 2016-07-29 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O envolvimento de receptores do tipo 5-HT3, assim como a rela??o do N?cleo
Dorsal da Rafe na modula??o da ansiedade vem sendo citados na literatura.
Levando em considera??o a grande parcela da popula??o que ? afetada pelos
transtornos de ansiedade, torna-se imprescind?vel a procura por novas formas
de tratamento mais eficientes e pelo aumento do conhecimento acerca do
assunto. Nesse sentido, o objetivo do presente estudo ? testar a hip?tese de
que receptores do tipo 5-HT3 presentes na por??o dorsal do n?cleo dorsal da
rafe (NDR) modulem as respostas relacionadas ? ansiedade em ratas. No
experimento 1, ratas Wistar com ? 90 dias de idade receberam tratamento (v.o)
de ondansetrona na dose de 0,1, 1 e 10 mg/kg/mL e, 60 minutos ap?s, foram
submetidas ao teste do Labirinto em T Elevado (LTE). 24 horas ap?s o LTE, os
animais receberam novamente a ondansetrona e foram submetidas ao teste do
Campo Aberto. Nos experimentos 2 e 3, as ratas foram submetidas ? cirurgia
estereot?xica para implanta??o de c?nula intra-NDR e receberam
administra??o local de salina ou dolasetrona nas doses de 100, 500 e
1000nmol (experimento 2) ou salina ou mCPBG nas doses de 2,5, 5 e 10?g
(experimento 3). Em ambos, 5 minutos ap?s a infus?o com a droga, os animais
foram submetidos ao teste do LTE, e 24 horas ap?s, receberam infus?o e
foram submetidos ao teste do Campo Aberto para obten??o de curvas doseresposta.
A ondansetrona na dose de 10mg/Kg/mL aumentou a lat?ncia de
fuga no LTE, sugerindo um efeito do tipo panicol?tico. A dolasetrona n?o
promoveu nenhum efeito no teste do LTE, em nenhuma das doses testadas. J?
o agonista mCPBG em todas as doses aumentou a lat?ncia de esquiva
inibit?ria em todas as tentativas, sugerindo um efeito do tipo ansiog?nico. Os
resultados obtidos no teste do Campo Aberto mostrou que n?o houve altera??o
na atividade locomotora das ratas causada pelos tratamentos. Os dados
obtidos refor?am a import?ncia dos receptores 5-HT3 no NDR na modula??o
da ansiedade. / The involvement of both 5-HT3 receptors and dorsal raphe nucleus in the
modulation of anxiety has been presented in the literature. Considering that
most of population is affected by anxiety disorders, it is essential to search for
new and more efficient forms of treatment and to increase the knowledge on the
subject. The aim of this study is to test the hypothesis that the 5-HT3 receptors
in the dorsal part of the dorsal raphe nucleus (dNDR) modulates the responses
related to anxiety in rats. In experiment one, female Wistar rats with ? 90 days
old were treated (p.o.) with ondansetron at doses of 0.1, 1 and 10 mg/kg/mL
and 60 minutes later, were submitted to the elevated T maze (ETM) test. 24
hours later, they received ondansetron and were submitted to the open Field
Test. In the experiments 2 and 3, the rats were submitted to the stereotactic
surgery for implantation of intra-NDR cannula and received administration (via
infusion) of saline or dolasetron at doses of 100, 500 and 1000nmol (experiment
2) or saline or mCPBG at doses of 2.5, 5 and 10mg (experiment 3). In both
experiments, 5 minutes after the infusion of the drug, the animals were
submitted to the test of ETM, and 24 hours after, they received drug infusion
and were submitted to the open field test, to obtain dose-response curves.
Ondansetron at a dose of 10mg/kg/mL increased latency of escape response in
ETM, suggesting an panicolytic-like effect. The dolasetron caused no effect in
ETM test in any of the tested doses. The agonist mCPBG instead, increased
the inhibitory avoidance latency in all doses, suggesting an anxiogenic-like
effect. Data obtained in the open field test showed no change in locomotor
activity of rats following the treatments. The data here obtained supports the
importance of 5-HT3 receptors in the NDR in the modulation of anxiety.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/22003 |
| Date | 29 July 2016 |
| Creators | Freire, B?rbara Thaise da Silva |
| Contributors | 28762725807, Gavioli, Elaine Cristina, 97013390968, Bertoglio, Leandro Jos?, 98761447900, Rachetti, Vanessa de Paula Soares |
| Publisher | PROGRAMA DE P?S-GRADUA??O EM CI?NCIAS BIOL?GICAS, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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