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Previous issue date: 2016-02-19 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Introdu??o: A endonuclease apur?nica/apurimid?nica (APE-1) ? uma prote?na essencial para a via do reparo por excis?o de bases (BER) do DNA, al?m de regula??o de atividades redox. A capacidade de c?lulas malignas em reconhecer e reparar danos no DNA ? um mecanismo importante para sobreviv?ncia tumoral, e estudos recentes sugerem que a superexpress?o da APE-1 pode se relacionar com o pobre progn?stico em alguns tumores. Objetivo: Analisar a imunoexpress?o da APE-1 em Adenomas Pleom?rficos (AP) e Carcinomas Ex-Adenomas Pleom?rficos (CaExAP) de gl?ndulas salivares. Materiais e M?todos: Foram selecionados 49 tumores fixados em formol e inclu?dos em parafina (33 AP e 16 CaExAP) que foram submetidos a estudo imuno-histoqu?mico pela t?cnica da imunoperoxidase. A imunoexpress?o da APE-1 foi avaliada de forma quantitativa pelo percentual de c?lulas imunopositivas. Para an?lise estat?stica foi adotado n?vel de signific?ncia de 5% (p ? 0,05). Resultados: Todos os casos de AP e CaExAP (n=49) foram positivos para APE-1, no entanto, houve maior express?o em CaExAP havendo diferen?a estatisticamente relevante (p<0,001). N?o foi encontrada associa??o da express?o da APE-1 entre tumores de gl?ndula salivar maior ou menor, entretanto, em AP n?o encapsulados (Mediana de express?o= 54,2%) houve maior express?o quando comparados a tumores encapsulados (p=0,02). A superexpress?o da APE-1 foi constatada principalmente em casos de CaExAP com met?stase linfonodal (Mediana de express?o= 90,3% - p=0,002) e padr?o invasivo (Mediana de express?o= 89,9% - p=0,003) quando comparados aos casos sem met?stase e intracapsulares. Conclus?o: Este estudo sugere que a APE-1 encontra-se desregulada nos tumores estudados. A maior express?o da APE-1 est? associada com a aus?ncia de c?psula completa em AP e a superexpress?o est? relacionada com o comportamento mais agressivo do CaExAP. / Introduction: Apurinic/Apyrimidinic Endonuclease 1 (APE-1) is an essential protein for DNA base excision repair (BER) pathway and regulation of redox activities. The ability of malignant cells to recognize and repair DNA damage is an important mechanism for tumor survival, and recent studies suggest that APE-1 overexpression is related to poor prognosis in some tumors. Purpose: To analyze the immunoreactivity of APE-1 in Pleomorphic Adenomas (PA) and Carcinomas Ex Pleomorphic Adenomas (CaExPA) of salivary glands. Materials and Methods: A total of 49 tumors fixed in formalin and embedded in paraffin (33 PA and 16 CaExPA) underwent immunohistochemical study by the immunoperoxidase technique. APE-1 immunoreactivity was evaluated quantitatively by the percentage of immunopositive cells. For statistical analysis a significance level of 5% (p? 0.05) was adopted. Results: All cases of PA and CaExPA (n=49) were positive for APE-1, however, there was a higher expression in CaExPA, with statistically significant difference (p<0.001). There was no association between APE-1 expression and tumors of major or minor salivary gland, however, not encapsulated PA (median expression = 54.2%) showed higher expression when compared to encapsulated tumors (p=0.02). APE-1 overexpression was found mainly in cases of CaExAP with lymph node metastasis (median expression = 90.3% - p=0.002) and invasive pattern (median expression = 89.9% - p=0.003), when compared to cases without metastasis and intracapsular pattern. Conclusion: This study suggests that APE-1 is deregulated in the studied tumors. The increased expression of APE-1 is associated with the absence of complete capsule in PA and it is associated with more aggressive behavior in CaExPA.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/20956 |
| Date | 19 February 2016 |
| Creators | Silva, Leorik Pereira da |
| Contributors | 67269621420, http://lattes.cnpq.br/5004397230198722, Souza, Lelia Batista de, 08588724472, http://lattes.cnpq.br/6671914892609743, Sobral, Ana Paula Veras, 74460536404, http://lattes.cnpq.br/7338874761603515, Barboza, Carlos Augusto Galv?o |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM PATOLOGIA ORAL, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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