Recent research in bioinformatics has presented multiple cell type identification meth- dologies using single cell RNA sequence data (scRNA-seq). However, a consensus on which cell typing methodology consistently demonstrates superior performance remains absent. Additionally, very few studies approach cell type identification through a semi- supervised learning study, whereby the information in unlabeled data is leveraged to train an enhanced classifier. This paper presents cell annotation methodologies through self- learning and graph-based semi-supervised learning, in both raw count scRNA-seq data as well as in a latent embedding. I find that a self-learning framework enhances perfor- mance compared to a solely supervised learning classifier. Additionally, modelling on the latent data representations consistently outperforms modelling on the original data. The results show an overall accuracy of 96.12%, whereas additional models achieve an average precision rate of 95.12% and an average recall rate of 94.40%. The semi-supervised learn- ing approaches in this thesis compare favourable to scANVI in terms of accuracy, average precision rate, average recall rate and average f1-score. Moreover, results for alternative scenarios, in which cell types among training and test data do not perfectly overlap, are reported in this thesis.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:liu-165996 |
| Date | January 2020 |
| Creators | Quast, Thijs |
| Publisher | Linköpings universitet, Statistik och maskininlärning |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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