The ability to control the physical environment at subcellular scales is critical to understanding cell and tissue behaviors regulated by extracellular interactions. However, open platform technology that allows one to create combinatorial physical environments is not readily available. This thesis describes the development of a low-cost system for creating complex hydrogel and ligand patterns using maskless lithography. Specifically, it incorporates light paths with interchangeable wavelengths to facilitate a broad range of chemistries. In addition, it also includes a motorized stage with an adaptable platform that can hold different conventional cell culture vessels. Finally, I have developed a LabVIEW interface that allows one to create repeating patterns across different wells quickly and easily. Taken together, this technology will enable more rapid probing of mechanobiological regulation for applications in tissue engineering, drug discovery, and developmental biology.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc2332551 |
| Date | 05 1900 |
| Creators | Monroy, Natanael F. |
| Contributors | Esmailie, Fateme, Yang, Huaxiao, Meckes, Brian |
| Publisher | University of North Texas |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | Text |
| Rights | Public, Monroy, Natanael F., Copyright, Copyright is held by the author, unless otherwise noted. All rights Reserved. |
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